Invitrogen

KIF11 Recombinant Rabbit Monoclonal Antibody (JU83-16)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (17) KIF11 antibodies

Datasheet

Protocols

Questions & Answers

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (4)
Advanced Verification (2)

FIGURE: 1 / 6

KIF11 Antibody (MA5-32792) in WB

Western Blot was performed using Anti-KIF11 Recombinant Rabbit Monoclonal Antibody (JU83-16) (Product # MA5-32792) and a 120 kDa band corresponding to Kinesin-like protein KIF11 was observed across cell lines tested. Nuclear enriched extracts (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), HEK-293 (Lane 3), Jurkat (Lane 4), FaDu (Lane 5), MDA-MB-231 (Lane 6), T-47D (Lane 7), U-2 OS (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose mem... View More

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KIF11 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Kinesin-like protein KIF11 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with KIF11 Recombinant Rabbit Monoclonal Antibody (JU83-16) (Product # MA5-32792) at 1:100 dilution (green channel) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature. Nuclei were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300). Merged image shows spindle poles localization o KIF11 in various cell cycle stages. The images were captured at 60X magnification.

KIF11 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Kinesin-like protein KIF11 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-KIF11 Recombinant Rabbit Monoclonal Antibody (JU83-16) (Product # MA5-32792). {KD}

Product Details

MA5-32792

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

1:10-1:50

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JU83-16

Immunogen

Recombinant protein within Human Eg5 aa 860-1,056

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2810068

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Kines; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5

Gene Aliases: EG5; HKSP; KIF11; KNSL1; MCLMR; TRIP5

UniProt ID: (Human) P52732

Entrez Gene ID: (Human) 3832

Molecular Function: microtubule binding motor protein

Function(s)

retrograde vesicle-mediated transport, Golgi to ER microtubule-based movement spindle organization mitotic spindle organization chromosome segregation mitotic nuclear division mitotic centrosome separation antigen processing and presentation of exogenous peptide antigen via MHC class II regulation of mitotic centrosome separation cell division mitotic spindle assembly

Process(es)

microtubule motor activity ATP binding microtubule binding ATP-dependent microtubule motor activity, plus-end-directed protein kinase binding protein complex binding