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Invitrogen
KLF5 Recombinant Rabbit Monoclonal Antibody (23H10L5)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
KLF5 Antibody (701885) in ICC/IF
For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous KLF5 using Anti- KLF5 Recombinant Rabbit Monoclonal Antibody (Product # 701885, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of KLF5 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938)... View More
Product Details
701885
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
23H10L5
Immunogen
Peptides corresponding to Human KLF5 (aa 11-24, 358-372)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633036
Product Specific Information
This antibody is predicted to react with Monkey, Rabbit and Pig
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. It contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. KLF5 is a transcription activator that promotes cell proliferation and oncogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: (intestinal Kruppel-like factor; basic transcription element binding protein 2; Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; colon kruppel-like factor; GC box binding protein 2; GC-box-binding protein 2; Intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; Klf5C isoform; Krueppel-like factor 5; Kruppel-like factor 5 (intestinal); RP11-505F3.5; Transcription factor BTEB2
Gene Aliases: BTEB2; CKLF; IKLF; KLF5
UniProt ID: (Human) Q13887
Entrez Gene ID: (Human) 688
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
transcription from RNA polymerase II promoter
microvillus assembly
regulation of microvillus assembly
skeletal muscle cell differentiation
positive regulation of fat cell differentiation
positive regulation of transcription from RNA polymerase II promoter
intestinal epithelial cell development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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