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KLHL1 Antibody (MA5-15700) in WB
Western blot analysis of KLHL1 was performed by loading 75 µg or 100 µg mouse (Ms) and rat (Rt) whole brain lysates per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked for 1 hour with TBST + 5% milk. The membrane was probed with a KLHL1 monoclonal antibody (Product # MA5-15700) at a dilution of 1:10,000 overnight at 16C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:2500 for 1 hour at room temperature. Detection was... View More
Product Details
MA5-15700
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3A8
Immunogen
Purified recombinant fragment of human KLHL1 expressed in E. Coli.
Conjugate
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_10984316
Product Specific Information
MA5-15700 targets KLHL1 in indirect ELISA, IF and WB applications and shows reactivity with Human, Mouse and Rat samples.
The MA5-15700 immunogen is purified recombinant fragment of human KLHL1 expressed in E. Coli.
MA5-15700 detects KLHL1 which has a predicted molecular weight of approximately 82kDa. In Western blot, MA5-15700 did not detect KLHL1 in whole rat brain lysates, but detected KLHL1 in hippocampal neuronal cell lysates.
Target Information
The mammalian Kelch-like 1 (KLHL1) was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues. The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin-organizing proteins. Based on the presence of anti-sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ30047; kelch-l like; kelch-like 1; kelch-like family member 1; Kelch-like protein 1; Mayven-related protein 2; RP11-394C23.1
Gene Aliases: KIAA1490; KLHL1; mKIAA1490; MRP2
UniProt ID: (Human) Q9NR64, (Mouse) Q9JI74
Entrez Gene ID: (Human) 57626, (Rat) 290426, (Mouse) 93688
Molecular Function:
scaffold/adaptor protein
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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