Western blot analysis of KLK7 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human KLK7-transfected 293T cells. Samples were loaded onto a 12% SDS-PAGE gel and probed with a KLK7 polyclonal antibody (Product # PA5-27252) at a dilution of 1:5000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 36 and 130 of Human KLK7|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27252 targets KLK7 in WB applications and shows reactivity with Human samples.
The PA5-27252 immunogen is synthetic peptide corresponding to a region within amino acids 36 and 130 of Human KLK7.
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded enzyme is thought to be involved in the proteolysis of intercellular cohesive structures preceding desquamation, which is the shedding of the outermost layer of the epidermis. Alternative splicing of this gene results in two transcript variants encoding the same protein.
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