Invitrogen

KLRG1 Monoclonal Antibody (2F1), Biotin, eBioscience™

Product Image Gallery

KLRG1 Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and 0.06 µg of Golden Syrian Hamster IgG Isotype Control (Product # 13-4914-81) (left) or 0.06 µg of Anti-Mouse KLRG1 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

Product Details

13-5893-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.06 µg/test	View 14 publications 14 publications

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Syrian hamster / IgG
Recommended Isotype Control	Syrian Hamster IgG Isotype Control, Biotin, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	2F1
Conjugate	Biotin Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 561 Alexa Fluor 647 APC APC-eFluor 780 eFluor 450 eFluor 506 FITC Functional Grade Pacific Orange PE PE-Cyanine5 PE-Cyanine5.5 PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702 Super Bright 780
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_466770

Product Specific Information

Description: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.

Applications Reported: 2F1 has been reported for use in flow cytometric analysis.

Applications Tested: The 2F1 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer.

Oncoimmunology

Huang RY,Francois A,McGray AR,Miliotto A,Odunsi K

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell responses.

The Journal of experimental medicine

Chen L,Christian DA,Kochanowsky JA,Phan AT,Clark JT,Wang S,Berry C,Oh J,Chen X,Roos DS,Beiting DP,Koshy AA,Hunter CA

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity.

Frontiers in immunology

Knolle MD,Chin SB,Rana BMJ,Englezakis A,Nakagawa R,Fallon PG,Git A,McKenzie ANJ

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2019

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

IL-15 signaling promotes adoptive effector T-cell survival and memory formation in irradiation-induced lymphopenia.

Cell & bioscience

Xu A,Bhanumathy KK,Wu J,Ye Z,Freywald A,Leary SC,Li R,Xiang J

13-5893 was used in Flow cytometry/Cell sorting to investigate the mechanism for adoptively transferred effector T-cell survival and memory formation.

Mon May 09 00:00:00 EDT 2016

Developmental Progression and Interrelationship of Central and Effector Regulatory T Cell Subsets.

Journal of immunology (Baltimore, Md. : 1950)

Toomer KH,Yuan X,Yang J,Dee MJ,Yu A,Malek TR

13-5893 was used in Flow cytometry/Cell sorting to elucidate the developmental progression and interrelationship of central and effector regulatory T cell subsets.

Sun May 01 00:00:00 EDT 2016

MHC-dependent inhibition of uterine NK cells impedes fetal growth and decidual vascular remodelling.

Nature communications

Kieckbusch J,Gaynor LM,Moffett A,Colucci F

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Fri Feb 28 00:00:00 EST 2014

Markers of nonselective and specific NK cell activation.

Journal of immunology (Baltimore, Md. : 1950)

Fogel LA,Sun MM,Geurs TL,Carayannopoulos LN,French AR

13-5893 was used in Flow cytometry/Cell sorting to demonstrate that a combination of Sca-1, CD27, and KLRG1 can distinguish NK cells based on their mechanism of activation.

Sat Jun 15 00:00:00 EDT 2013

Role for E-cadherin as an inhibitory receptor on epidermal gammadelta T cells.

Journal of immunology (Baltimore, Md. : 1950)

Uchida Y,Kawai K,Ibusuki A,Kanekura T

13-5893 was used in Flow cytometry/Cell sorting to analyse functional differences between E-cadherin and α(E)β(7) integrin expressed on murine dendritic epidermal T cells.

Wed Jun 15 00:00:00 EDT 2011

One naive T cell, multiple fates in CD8+ T cell differentiation.

The Journal of experimental medicine

Gerlach C,van Heijst JW,Swart E,Sie D,Armstrong N,Kerkhoven RM,Zehn D,Bevan MJ,Schepers K,Schumacher TN

13-5893 was used in Flow cytometry/Cell sorting to assess the potential for development of single naive T cells into different subsets.

Mon Jun 07 00:00:00 EDT 2010

IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response.

Blood

Rubinstein MP,Lind NA,Purton JF,Filippou P,Best JA,McGhee PA,Surh CD,Goldrath AW

13-5893 was used in Flow cytometry/Cell sorting to study IL-7 and IL-15 regulation of CD8+ T-cell subsets during contraction of the immune response.

Sat Nov 01 00:00:00 EDT 2008

Homeostasis and effector function of lymphopenia-induced "memory-like" T cells in constitutively T cell-depleted mice.

Journal of immunology (Baltimore, Md. : 1950)

Voehringer D,Liang HE,Locksley RM

13-5893-82 was used in Flow Cytometry to demonstrate that T(ML) cells are not sufficient to mediate an effective immune response in constitutively T cell-depleted mice.

Tue Apr 01 00:00:00 EDT 2008

Mechanisms involved in synergistic anticancer immunity of anti-4-1BB and anti-CD4 therapy.

Cancer research

Choi BK,Kim YH,Kang WJ,Lee SK,Kim KH,Shin SM,Yokoyama WM,Kim TY,Kwon BS

13-5893 was used in Flow cytometry/Cell sorting to demonstrate a therapy that combines the use of two mAbs for the enhanced production of efficient tumour-killing cytotoxic T lymphocytes.