|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 386-415 amino acids from the C-terminal region of human KRT4|
|Purification||Ammonium sulfate precipitation, Size-exclusion - Dialysis|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
The protein is a member of the keratin family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Intestinal epithelial culture under an air-liquid interface: a tool for studying human and mouse esophagi.
PA5-13684 was used in immunohistochemistry - paraffin section to describe a method to maintain mouse and human esophagi ex vivo
|Yokobori T,Suzuki S,Miyazaki T,Sohda M,Sakai M,Tanaka N,Ozawa D,Hara K,Honjo H,Altan B,Fukuchi M,Ishii H,Iwatsuki M,Sugimachi K,Sudo T,Iwaya T,Nishida N,Mimori K,Kuwano H,Mori M||Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus (29:843)||2016|