|Tested species reactivity||Virus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of KSHV ORF45 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14769 targets KSHV ORF45 in IHC and WB applications and shows reactivity with Virus samples.
The MA5-14769 immunogen is purified recombinant fragment of KSHV ORF45 expressed in E. Coli.
Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi and quote;s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman and quote;s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.