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Western blot analysis of immunoglobulin (Ig) kappa light chain was performed by loading the indicated amounts of purified human IgG/kappa and 10ul of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temperature. Ig kappa light chain was detected at ~25kD using a biotinylated mouse anti-human Ig kappa light chain monoclonal antibody (Product # MA5-12114) diluted to a concentration of 0.5ug/ml in blocking buffer, overnight at 4C overnight on a rocking platform. The blot was washed in TBST, and probed with Streptavidin-HRP (Product # 21126) at a dilution of 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). Note: IgG heavy chain (~55kD) was also detected when larger amounts of purified IgG were loaded per lane.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||B lymphoma cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Target||Kappa Light Chain|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1 µg/ml|
|Flow Cytometry (Flow)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400-1:800|
|Western Blot (WB)||1:400-1:1600|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
By Western blot, MA5-12114 detects Human Ig kappa light chain chain at ~25kD in human samples. At high concentrations, the antibody will also detect heavy chain.
By ELISA and WB, MA5-12114 does not react with human Ig lambda light chain.
Antibody to the kappa light chain of immunoglobulin is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Liquid-based cytologic findings of solitary extramedullary plasmacytoma in thyroid: a case report identified with fine-needle aspiration cytology.
MA5-12114 was used in immunocytochemistry to report on a case of solitary extramedullary plasmacytoma of the thyroid
|Lee CH,Jung YY,Chung YR,Ryu HS||Diagnostic cytopathology (42:964)||2014|
Composite lymphoma in the anterior mediastinum: a case report and review of the literature.
MA5-12114 was used in immunohistochemistry to present a case report of an unusual composite lymphoma in the mediastinum
|Yu G,Kong L,Qu G,Zhang Q,Wang W,Jiang L||Diagnostic pathology (6:null)||2011|
|Not Applicable||Not Cited||
Extramedullary plasmacytoma: clinicopathology, immunohistochemistry and therapeutic approach to a case with a tonsillar site.
MA5-12114 was used in immunohistochemistry to report a clinical case of extramedullary plasmacytoma
|Tokatli F,Puyan FO,Alas RC,Tuncbilek N,Uzal C||Hematology/oncology and stem cell therapy (1:241)||2010|
|Not Applicable||Not Cited||
Suppression of human melanoma tumor growth in SCID mice by a human high molecular weight-melanoma associated antigen (HMW-MAA) specific monoclonal antibody.
MA5-12114 was used in blocking or activating experiment to study the ability of a HMW-MAA monoclonal antibody to suppress human melanoma tumor growth in SCID mice
|Hafner C,Breiteneder H,Ferrone S,Thallinger C,Wagner S,Schmidt WM,Jasinska J,Kundi M,Wolff K,Zielinski CC,Scheiner O,Wiedermann U,Pehamberger H||International journal of cancer (114:426)||2005|