|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human Keratin 14|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Staining of formalin-fixed tissues requires digesting tissue sections with Pepsin for 10 min at 37°C, followed by incubation with primary antibody for 10 min at room temperature. A suggested positive control is skin or squamous cell carcinoma.
Keratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5. Keratin 14 has been studied as a prognostic marker in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.