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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human keratin 14 protein|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:25|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skin, squamous cell carcinoma or prostate tissue.
Keratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CK-14; CK14; cytokeratin 14; cytokeratin-14; Dowling-Meara; EBS3; EBS4; keratin; keratin 14 epidermolysis bullosa simplex; keratin 14, type I; keratin-14; Koebner; NFJ; type I cytoskeletal 14
CK14; EBS3; EBS4; K14; KRT14; NFJ