|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human cytokeratin 5|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skin, breast, prostate or mesothelioma.
Twnety human keratins are divided in to acidic (pl<5.7) and basic (pl > 6.0) subfamilies. Members of the acidic and basic subfamlilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown usefulness of keratins as markers in cancer research and tumor identification. Point mutations in cytokeratin5 gene can cause various types of epidermolysis bullosa simplex. It is expressed in most epithelial and biphasic mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells.
PA1-37974 was used in immunocytochemistry to investigate factors involved in the crosstalk between basal cells and endothelial cells.
|Ding BS,Gomi K,Rafii S,Crystal RG,Walters MS||Journal of cell science (128:2983)||2015|
Activation of NOTCH1 or NOTCH3 signaling skews human airway basal cell differentiation toward a secretory pathway.
PA1-37974 was used in immunocytochemistry and western blot to investigate the role of Notch signaling in human basal cell differentiation
|Gomi K,Arbelaez V,Crystal RG,Walters MS||PloS one (10:null)||2015|
Activation of soluble guanylyl cyclase prevents foam cell formation and atherosclerosis.
PA1-37974 was used in western blot to study the mechanism by which soluble guanylyl cyclase activation blocks macrophage foam cell formation and atherosclerosis
|Tsou CY,Chen CY,Zhao JF,Su KH,Lee HT,Lin SJ,Shyue SK,Hsiao SH,Lee TS||Acta physiologica (Oxford, England) (210:799)||2014|
Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity.
PA1-37974 was used in western blot to generate an immortalized human epithelial basal cell line capable of multipotent differentiation
|Walters MS,Gomi K,Ashbridge B,Moore MA,Arbelaez V,Heldrich J,Ding BS,Rafii S,Staudt MR,Crystal RG||Respiratory research (14:null)||2013|