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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from C-terminus of human cytokeratin 5.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skin, breast, prostate, prostate carcinoma or mesothelioma.
Twenty human keratins are divided into acidic (pI <5.7) and basic (pI >6.0) subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor identification. Point mutations in cytokeratin 5 gene can cause various types of epidermolysis bullosa simplex. It is expressed in most epithelial and biphasic mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunohistochemistry in the diagnosis of papillary lesions of the breast.
MA5-16372 was used in immunohistochemistry to establish a diagnostic immunohistochemical marker panel applicable to breast papilliary lesions
|Tse GM,Ni YB,Tsang JY,Shao MM,Huang YH,Luo MH,Lacambra MD,Yamaguchi R,Tan PH||Histopathology (65:839)||2014|
B-cell maturation antigen (BCMA) activation exerts specific proinflammatory effects in normal human keratinocytes and is preferentially expressed in inflammatory skin pathologies.
MA5-16372 was used in immunocytochemistry to study the expression and role of B-cell maturation antigen in inflammatory skin pathologies
|Alexaki VI,Pelekanou V,Notas G,Venihaki M,Kampa M,Dessirier V,Sabour-Alaoui S,Stathopoulos EN,Tsapis A,Castanas E||Endocrinology (153:739)||2012|
58 kDa cytokeratin; CK-5; CK5; Cytokeratin-5; DDD; Dowling-Meara/Kobner/Weber-Cockayne types; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K5; keratin; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5 epidermolysis bullosa simplex; keratin 5, type II; Keratin, type II cytoskeletal 5; Keratin-5; KRT5A; type II cytoskeletal 5; Type-II keratin Kb5
CK5; DDD; DDD1; EBS2; K5; KRT5; KRT5A