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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human keratin 6 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is prostate or skin tissue.
Keratins 6 and 16 are expressed in keratinocytes, which undergo rapid turnover in the suprabasal region (also known as hyperproliferation related keratins). Keratin 6 (CK6) is found in hair follicles, neck squamous cell carcinomas, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CK-6C; CK-6E; cytokeratin-6C; cytokeratin-6E; K6C; K6E; keratin; keratin 6C, type II; keratin 6E; keratin K6h; keratin-6C; KRT6E; type II cytoskeletal 6C; type-II keratin Kb12
K6E; KRT6C; KRT6E; PPKNEFD