|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytokeratin purified from human breast carcinoma cell line MC-7.|
|Storage buffer||tissue culture supernatant with 1% FBS|
|Contains||10mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:5-1:25|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Keratin 8 phosphorylation regulates its transamidation and hepatocyte Mallory-Denk body formation.
MA1-35565 was used in western blot to study the role of keratin 8 phosphorylation in regulating Mallory-Denk body formation
|Kwan R,Hanada S,Harada M,Strnad P,Li DH,Omary MB||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (26:2318)||2012|
Analysis of keratin polypeptides 8 and 19 variants in inflammatory bowel disease.
MA1-35565 was used in western blot to study whether variants of keratin 8 and keratin 19 are associated with inflammatory bowel disease
|Tao GZ,Strnad P,Zhou Q,Kamal A,Zhang L,Madani ND,Kugathasan S,Brant SR,Cho JH,Omary MB,Duerr RH||Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association (5:857)||2007|
Protein phosphatase-2A associates with and dephosphorylates keratin 8 after hyposmotic stress in a site- and cell-specific manner.
MA1-35565 was used in western blot to study the site-specific dephosphorylation of keratin 8 by protein phosphatase-2A and how this varies according to cell type
|Tao GZ,Toivola DM,Zhou Q,Strnad P,Xu B,Michie SA,Omary MB||Journal of cell science (119:1425)||2006|
Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies.
MA1-35565 was used in western blot to produce and characterize human disease-related anti-keratin rabbit monoclonal antibodies
|Tao GZ,Nakamichi I,Ku NO,Wang J,Frolkis M,Gong X,Zhu W,Pytela R,Omary MB||Experimental cell research (312:411)||2006|
Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease.
MA1-35565 was used in western blot to study keratin 8 and keratin 18 hyperphosphorylation as markers for progression of human liver disease
|Toivola DM,Ku NO,Resurreccion EZ,Nelson DR,Wright TL,Omary MB||Hepatology (Baltimore, Md.) (40:459)||2004|
Keratin 8 mutations in patients with cryptogenic liver disease.
MA1-35565 was used in western blot to study mutations of keratin 8 in cryptogenic liver disease patients
|Ku NO,Gish R,Wright TL,Omary MB||The New England journal of medicine (344:1580)||2001|
Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas.
MA1-35565 was used in immunocytochemistry to investigate the transdifferentiation of human embryonic pancreatic cells to hepatocytes
|Sumitran-Holgersson S,Nowak G,Thowfeequ S,Begum S,Joshi M,Jaksch M,Kjaeldgaard A,Jorns C,Ericzon BG,Tosh D||Cell transplantation (18:183)||2009|
Antigen expression of human eccrine sweat glands.
MA1-35565 was used in immunohistochemistry to identify the phenotypic markers of human eccrine sweat glands
|Li HH,Zhou G,Fu XB,Zhang L||Journal of cutaneous pathology (36:318)||2009|
Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.
MA1-35565 was used in immunohistochemistry to study two adult patients with mesenchymal hamartoma of the liver
|Yesim G,Gupse T,Zafer U,Ahmet A||Journal of hepato-biliary-pancreatic surgery (12:502)||2005|