|Tested species reactivity||Human|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human keratin 8.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.
Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in association with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia. It is present in hepatocellular carcinomas and the majority of adenocarcinomas and ductal carcinomas, but it is absent in squamous cell carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Genetic deletion of caspase-2 accelerates MMTV/c-neu-driven mammary carcinogenesis in mice.
MA5-16420 was used in immunohistochemistry to examine the possible role of caspase-2 as a tumor suppressor in the mouse mammary carcinogenesis model
|Parsons MJ,McCormick L,Janke L,Howard A,Bouchier-Hayes L,Green DR||Cell death and differentiation (20:1174)||2013|
A small molecule inhibitor of SRC family kinases promotes simple epithelial differentiation of human pluripotent stem cells.
MA5-16420 was used in flow cytometry and immunocytochemistry to describe conditions that promote development of hPSC-derived simple epithelial cells
|Lian X,Selekman J,Bao X,Hsiao C,Zhu K,Palecek SP||PloS one (8:null)||2013|