Invitrogen

Ki-67 Polyclonal Antibody

Product Image Gallery

Ki-67 Antibody — Figure 2 Mangiferin regulates islet regeneration. (A) Representative immunohistochemical images of insulin-positive cells (red) and BrdU-labeled cells (brown) in the different groups. Arrows indicate the BrdU-labeled cells (scale bar, 100 u m). (B-D) Percentages of (B) BrdU-positive, (C) Ki67-positive and (D) PCNA-positive among insulin-positive beta-cells in the different groups were calculated. At least 10 islets containing at least 1,000 beta-cells were counted per mouse (n=6). (E) Comparison of remnant pancreas weights. (F) Relative beta-cell volume determined by point counting. (G) beta-cell mass calculated by multiplying the relative beta-cell volume by the total weight of remnant pancreas. Two or three slides (200 u m apart) from the broadest pancreatic sections were subjected to beta-cell mass measurement (n=8 for each group). Values are expressed as the mean +- standard error of the mean. * P<0.05 and ** P<0.01. BrdU, bromodeoxyuridine; PPx, partial pancreatectomy; PCNA, proliferating cell nuclear antigen.

Ki-67 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of Ki67 in mouse lung tissue using a Ki67 polyclonal antibody (Product # PA1-21520) at a dilution of 1:50.

Ki-67 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Ki67 in formalin-fixed, paraffin-embedded human tonsil tissue using a Ki67 polyclonal antibody (Product # PA1-21520) and detected using ABC method with AEC chromogen.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

Assay Dependent

Immunofluorescence (IF)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

10-20 µg/mL

Published Applications

Immunohistochemistry (IHC)

See 4 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Immunocytochemistry (ICC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide from the human Ki-67 antigen.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

10mM PBS, pH 7.4, with 0.2% BSA

Contains

0.1% sodium azide

Storage conditions

4° C, do not freeze

RRID

AB_559806

Product Specific Information

PA1-21520 detects Ki67 in human, mouse, and rat samples.

PA1-21520 has been successfully used in immunohistochemistry (paraffin) and ICC/IF procedures. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH6.0, for 10-20 minutes, followed by coolong at room temperature for 20 minutes.

The PA1-21520 immunogen is a synthetic peptide from the human Ki-67 antigen. PA1-21520 recognizes an epiptope in the middle of the Ki67 protein.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105

Gene Aliases: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

UniProt ID: (Human) P46013, (Mouse) E9PVX6

Entrez Gene ID: (Human) 4288, (Rat) 291234, (Mouse) 17345

Function(s)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

Process(es)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding molecular_function