Invitrogen

Ki-67 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

8 References

View all (153) Ki-67 antibodies

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Datasheet

Protocols

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Ki-67 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Ki67 was performed using 70% confluent log phase HeLa cells serum starved for 36 hours followed by serum release for 6 hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Ki67 Polyclonal Antibody (Product # PA1-21520) at 10 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415 , 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the serum starved cells with reduced signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Ki-67 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil using Ki-67 Polyclonal Antibody (Product # PA1-21520). Signal is detected using ABC method with AEC chromogen.

Ki-67 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of HT-29 xenograft tumor in mouse 20x pretreatment w/ citrate pH 6.0 using anti-Ki-67 Polyclonal Antibody (Product # PA1-21520) at 5 µg/mL for 30 min at RT.

Ki-67 Antibody — Detection of altered expression of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of Ki67 using Ki67 Polyclonal Antibody (Product # PA1-21520), shows decreased expression of Ki67 in HeLa cell line upon serum starvation for 36 hours. Serum starvation followed by serum release for 6 hours resulted in increase in Ki67 expression in the nucleus of HeLa cells. {TM}

Ki-67 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P)) — Fig. 1 Intravenous bleomycin induces subpleural fibrosis associated with pleural cell differentiation and migration. a Histological analysis of lung sections from mice receiving either NaCl or BLM intravenously at D14 and D21. Representative images (insert: NaCl at the corresponding time point. Scale bars : 500 mum). b Ashcroft scoring of lung sections. c Profiles of Picrosirius Red signal according to distance to the pleura at D3, D14 and D21. d Area Under Curve comparison of areas targeting the pleura ( left ) or subpleural ( right ) areas at D3, D14 and D21. For collagen quantification, data are represented as mean of Picrosirius Red signal normalized to the NaCl condition at the corresponding time point. Data expressed as mean +- SEM. n = 4 for NaCl groups, n = 6 for BLM groups at D3 and D21, and n = 6 for NaCl and n = 8 for BLM at D14. e Representative images of immunostaining for HSP27, alphaB-crystallin and KI-67 of lung sections from mice receiving NaCl or BLM at D14 (arrows indicate positive cells. Counterstaining: Harris hematoxylin. Scale bars : 100 mum). f Levels of proMMP-9 and active MMP-2 in PLF was determined by western blot. Representative results from NaCl ( n = 3) or BLM ( n = 3) treated mice at D14 are shown. g AdLacZ-labeled pleural cells were stained for beta-galactosidase activity ( blue staining ) in lung section from mice receiving intravenously NaCl or BLM at D5 or D10. Representative images are shown ( n = 4 NaCl, n = 6 BLM. Scale bars: 200 mum), In

Product Details

PA1-21520

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	10-20 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	10 µg/mL	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide from the human Ki-67 antigen.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	10mM PBS with 0.2% BSA
Contains	0.1% sodium azide
Storage conditions	4° C, do not freeze
RRID	AB_559806

Product Specific Information

Recommended positive controls: Tonsil or Breast carcinoma.

Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH6.0, for 10-20 minutes, followed by coolong at room temperature for 20 minutes.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibiting SUMO1-mediated SUMOylation induces autophagy-mediated cancer cell death and reduces tumour cell invasion via RAC1.

Journal of cell science

Lorente M,García-Casas A,Salvador N,Martínez-López A,Gabicagogeascoa E,Velasco G,López-Palomar L,Castillo-Lluva S

Published figure using Ki-67 polyclonal antibody (Product # PA1-21520) in Immunofluorescence

Tue Oct 22 00:00:00 EDT 2019

Mangiferin induces islet regeneration in aged mice through regulating p16INK4a.

International journal of molecular medicine

Wang H,He X,Lei T,Liu Y,Huai G,Sun M,Deng S,Yang H,Tong R,Wang Y

Published figure using Ki-67 polyclonal antibody (Product # PA1-21520) in Immunohistochemistry

Fri Jun 01 00:00:00 EDT 2018

Dual Inhibition of CDK4 and CDK2 via Targeting p27 Tyrosine Phosphorylation Induces a Potent and Durable Response in Breast Cancer Cells.

Molecular cancer research : MCR

Patel P,Tsiperson V,Gottesman SRS,Somma J,Blain SW

PA1-21520 was used in Western Blotting to demonstrate that CDK2 compensates for loss of CDK4 activity to rescue palbociclib-arrested breast cancer cells, suggesting that inhibition of both kinases is required to achieve durable response.

Thu Mar 01 00:00:00 EST 2018

Pleural inhibition of the caspase-1/IL-1β pathway diminishes profibrotic lung toxicity of bleomycin.

Respiratory research

Burgy O,Bellaye PS,Causse S,Beltramo G,Wettstein G,Boutanquoi PM,Goirand F,Garrido C,Bonniaud P

PA121520 was used in immunohistochemistry - paraffin section to investigate the role of IL-1beta/caspase-1 signaling in bleomycin lung toxicity and in pleural mesothelial cell transformation

Tue Nov 29 00:00:00 EST 2016

Pleural inhibition of the caspase-1/IL-1β pathway diminishes profibrotic lung toxicity of bleomycin.

Respiratory research

Burgy O,Bellaye PS,Causse S,Beltramo G,Wettstein G,Boutanquoi PM,Goirand F,Garrido C,Bonniaud P

PA121520 was used in immunohistochemistry - paraffin section to investigate the role of IL-1beta/caspase-1 signaling in bleomycin lung toxicity and in pleural mesothelial cell transformation

Tue Nov 29 00:00:00 EST 2016

Fat-1 gene inhibits human oral squamous carcinoma cell proliferation through downregulation of β-catenin signaling pathways.

Experimental and therapeutic medicine

Nie D,Wang Z,Zhang Y,Pang D,Ouyang H,Li LI

Published figure using Ki-67 polyclonal antibody (Product # PA1-21520) in Immunohistochemistry

Fri Jan 01 00:00:00 EST 2016

Tumor bioengineering using a transglutaminase crosslinked hydrogel.

PloS one

Fang JY,Tan SJ,Yang Z,Tayag C,Han B

PA1-21520 was used in immunocytochemistry to develop a 3D culturing system to mimic the tumor microenvironment using transglutaminase-cross-linked gelatin gel (Col-Tgel)

Mon Dec 07 00:00:00 EST 2015

Minnelide reduces tumor burden in preclinical models of osteosarcoma.

Cancer letters

Banerjee S,Thayanithy V,Sangwan V,Mackenzie TN,Saluja AK,Subramanian S

PA1-21520 was used in immunohistochemistry to study the beneficial effects of minnelide on tumor burden and lung metastasis in osteosarcoma models

Sun Jul 28 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2

Gene Aliases: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

UniProt ID: (Human) P46013, (Mouse) E9PVX6

Entrez Gene ID: (Human) 4288, (Rat) 291234, (Mouse) 17345

Function(s)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

Process(es)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding molecular_function