Invitrogen

Ki-67 Polyclonal Antibody

Ki-67 Antibody in Immunocytochemistry (ICC/IF)

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Ki-67 Antibody (PA1-21520) in ICC/IF

Immunofluorescence analysis of Ki67 was performed using 70% confluent log phase HeLa cells serum starved for 36 hours followed by serum release for 6 hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Ki67 Polyclonal Antibody (Product # PA1-21520) at 10 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Seco... View More

Ki-67 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ki-67 Antibody in Immunohistochemistry (IHC)

Ki-67 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

Product Image Gallery

Ki-67 Antibody — Detection of altered expression of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of Ki67 using Ki67 Polyclonal Antibody (Product # PA1-21520), shows decreased expression of Ki67 in HeLa cell line upon serum starvation for 36 hours. Serum starvation followed by serum release for 6 hours resulted in increase in Ki67 expression in the nucleus of HeLa cells. {TM}

Product Details

PA1-21520

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

10-20 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

10 µg/mL

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide from the human Ki-67 allantigen.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 0.2% BSA

Contains

0.1% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_559806

Product Specific Information

Recommended positive controls: Tonsil or Breast carcinoma.

Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH6.0, for 10-20 minutes, followed by coolong at room temperature for 20 minutes.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The beneficial role of the synthetic microneurotrophin BNN20 in a focal demyelination model.

Journal of neuroscience research

Kalafatakis I,Patellis A,Charalampopoulos I,Gravanis A,Karagogeos D

PA1-21520 was used in Immunocytochemistry, Immunohistochemistry (Paraffin) to develop blood-brain barrier-permeable synthetic agonists of neurotrophin receptors that could reduce inflammation, protect and increase the number of functional OLs by promoting their differentiation/maturation.

Sat May 01 00:00:00 UTC 2021

Inhibiting SUMO1-mediated SUMOylation induces autophagy-mediated cancer cell death and reduces tumour cell invasion via RAC1.

Journal of cell science

Lorente M,García-Casas A,Salvador N,Martínez-López A,Gabicagogeascoa E,Velasco G,López-Palomar L,Castillo-Lluva S

Published figure using Ki-67 polyclonal antibody (Product # PA1-21520) in Immunofluorescence

Tue Oct 22 00:00:00 UTC 2019

Dual Inhibition of CDK4 and CDK2 via Targeting p27 Tyrosine Phosphorylation Induces a Potent and Durable Response in Breast Cancer Cells.

Molecular cancer research : MCR

Patel P,Tsiperson V,Gottesman SRS,Somma J,Blain SW

PA1-21520 was used in Western Blotting to demonstrate that CDK2 compensates for loss of CDK4 activity to rescue palbociclib-arrested breast cancer cells, suggesting that inhibition of both kinases is required to achieve durable response.

Thu Mar 01 00:00:00 UTC 2018

Mangiferin induces islet regeneration in aged mice through regulating p16INK4a.

International journal of molecular medicine

Wang H,He X,Lei T,Liu Y,Huai G,Sun M,Deng S,Yang H,Tong R,Wang Y

Published figure using Ki-67 polyclonal antibody (Product # PA1-21520) in Immunohistochemistry

Fri Jun 01 00:00:00 UTC 2018

Fat-1 gene inhibits human oral squamous carcinoma cell proliferation through downregulation of β-catenin signaling pathways.

Experimental and therapeutic medicine

Nie D,Wang Z,Zhang Y,Pang D,Ouyang H,Li LI

Published figure using Ki-67 polyclonal antibody (Product # PA1-21520) in Immunohistochemistry

Fri Jan 01 00:00:00 UTC 2016

Pleural inhibition of the caspase-1/IL-1β pathway diminishes profibrotic lung toxicity of bleomycin.

Respiratory research

Burgy O,Bellaye PS,Causse S,Beltramo G,Wettstein G,Boutanquoi PM,Goirand F,Garrido C,Bonniaud P

Published figure using Ki-67 polyclonal antibody (Product # PA1-21520) in Immunohistochemistry

Tue Nov 29 00:00:00 UTC 2016

Pleural inhibition of the caspase-1/IL-1β pathway diminishes profibrotic lung toxicity of bleomycin.

Respiratory research

Burgy O,Bellaye PS,Causse S,Beltramo G,Wettstein G,Boutanquoi PM,Goirand F,Garrido C,Bonniaud P

Published figure using Ki-67 polyclonal antibody (Product # PA1-21520) in Immunohistochemistry

Tue Nov 29 00:00:00 UTC 2016

Tumor bioengineering using a transglutaminase crosslinked hydrogel.

PloS one

Fang JY,Tan SJ,Yang Z,Tayag C,Han B

PA1-21520 was used in immunocytochemistry to develop a 3D culturing system to mimic the tumor microenvironment using transglutaminase-cross-linked gelatin gel (Col-Tgel)

Mon Dec 07 00:00:00 UTC 2015

Minnelide reduces tumor burden in preclinical models of osteosarcoma.

Cancer letters

Banerjee S,Thayanithy V,Sangwan V,Mackenzie TN,Saluja AK,Subramanian S

PA1-21520 was used in immunohistochemistry to study the beneficial effects of minnelide on tumor burden and lung metastasis in osteosarcoma models

Sun Jul 28 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2

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Gene Aliases: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

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UniProt ID: (Human) P46013, (Mouse) E9PVX6

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Entrez Gene ID: (Human) 4288, (Rat) 291234, (Mouse) 17345

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Function(s)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding molecular_function

Process(es)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ki-67 Polyclonal Antibody

Ki-67 Antibody (PA1-21520) in ICC/IF

Product Details

PA1-21520

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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