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Immunohistochemistry analysis of Ki-67 showing staining in the nucleus of paraffin-embedded human tonsil tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Ki-67 Rabbit Polyclonal Antibody (PA516446) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the human Ki67 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16446 targets Ki-67 in IHC (P) applications and shows reactivity with Rat and Human samples.
The PA5-16446 immunogen is a synthetic peptide from the human Ki67 protein.
Ki67 is a nuclear protein, which is expressed in the proliferating cells. Ki67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiscent) phase are negative for this protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Oncogenic Actions of the Nuclear Receptor Corepressor (NCOR1) in a Mouse Model of Thyroid Cancer.
PA5-16446 was used in immunohistochemistry to use a murine model of thyroid cancer to study the oncogenic activity of NCOR1
|Fozzatti L,Park JW,Zhao L,Willingham MC,Cheng SY||PloS one (8:null)||2016|
The use of mouse models of breast cancer and quantitative image analysis to evaluate hormone receptor antigenicity after microwave-assisted formalin fixation.
PA5-16446 was used in immunohistochemistry to study hormone receptor immunogenicity following the use of rapid microwave-assisted formalin fixation of samples from murine models of breast cancer
|Engelberg JA,Giberson RT,Young LJ,Hubbard NE,Cardiff RD||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (62:319)||2014|
CXCR4, but not CXCR7, discriminates metastatic behavior in non-small cell lung cancer cells.
PA5-16446 was used in immunohistochemistry to study non-small cell lung cancer metastasis and the relative contributions of CXCR4 and CXCR7
|Choi YH,Burdick MD,Strieter BA,Mehrad B,Strieter RM||Molecular cancer research : MCR (12:38)||2014|
Silencing MED1 sensitizes breast cancer cells to pure anti-estrogen fulvestrant in vitro and in vivo.
PA5-16446 was used in immunohistochemistry to study the increased sensitivity of breast cancer cells to the estrogen receptor antagonist fulvestrant following knockdown of MED1
|Zhang L,Cui J,Leonard M,Nephew K,Li Y,Zhang X||PloS one (8:null)||2013|
Dietary selenium deficiency exacerbates DSS-induced epithelial injury and AOM/DSS-induced tumorigenesis.
PA5-16446 was used in immunohistochemistry to study the mechanisms underlying the deleterious effects of selenium deficiency on experimental colitis and tumorigenesis in rat models
|Barrett CW,Singh K,Motley AK,Lintel MK,Matafonova E,Bradley AM,Ning W,Poindexter SV,Parang B,Reddy VK,Chaturvedi R,Fingleton BM,Washington MK,Wilson KT,Davies SS,Hill KE,Burk RF,Williams CS||PloS one (8:null)||2013|
Reduced androgen receptor expression accelerates the onset of ERBB2 induced breast tumors in female mice.
PA5-16446 was used in immunohistochemistry to study a murine model of ERBB2-induced breast tumor and the role of reduced androgen receptor levels in promoting malignant transformation
|Hodgson MC,Vanostran G,Alghamdi S,Poppiti RJ,Agoulnik AI,Agoulnik IU||PloS one (8:null)||2013|
Initiation of prostate cancer in mice by Tp53R270H: evidence for an alternative molecular progression.
PA5-16446 was used in immunohistochemistry to study the role of the R270H mutant form of Tp53 in the initiation of prostate cancer in a murine model
|Vinall RL,Chen JQ,Hubbard NE,Sulaimon SS,Shen MM,Devere White RW,Borowsky AD||Disease models & mechanisms (5:914)||2012|
Zbed3 contributes to malignant phenotype of lung cancer via regulating β-catenin and P120-catenin 1.
PA5-16446 was used in immunohistochemistry - paraffin section to investigate the role of Zbed3 in malignant phenotype of lung cancer
|Fan C,Jiang G,Zhang X,Miao Y,Lin X,Luan L,Xu Z,Zhang Y,Zhao H,Liu D,Wang E||Molecular carcinogenesis (54 Suppl 1:E138)||2015|
Curcumin: a unique antioxidant offers a multimechanistic approach for management of hepatocellular carcinoma in rat model.
PA5-16446 was used in immunohistochemistry - paraffin section to investigate the role of curcumin as a protective agent against hepatocellular carcinoma in rats
|Ahmed HH,Shousha WG,Shalby AB,El-Mezayen HA,Ismaiel NN,Mahmoud NS||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (36:1667)||2015|
antigen identified by monoclonal antibody Ki-67; antigen KI-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105
KIA; MIB-; MIB-1; MKI67; PPP1R105