Invitrogen

Ki-67 Monoclonal Antibody (SolA15), eFluor™ 450, eBioscience™

Product Image Gallery

Ki-67 Antibody in Flow Cytometry (Flow) — C57Bl/6 splenocytes were unstimulated (left) or stimulated for 2 days with Anti-Mouse CD3 Functional Grade Purified (Product # 16-0031-82) (right). Cells were surface stained with Anti-Mouse CD19 FITC (Product # 11-0193-82) then fixed and permeabilized with the Foxp3 Staining Buffer Set (Product # 00-5523-00) and intracellularly stained with 0.06 µg of Anti-Mouse/Rat Ki-67 eFluor® 450. Total viable cells, as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), were used for analysis.

Product Details

48-5698-82

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
Flow Cytometry (Flow)	0.125 µg/test	View 20 publications 20 publications
Functional Assay (FN)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat
Published species	Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	SolA15
Conjugate	eFluor™ 450 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 660 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 737 Brilliant UV 805 eFluor 506 eFluor 570 eFluor 615 eFluor 660 FITC PE PE-Cyanine5 PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_11149124

Product Specific Information

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).

The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.

Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This SolA15 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523). This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Lymphatic PD-L1 Expression Restricts Tumor-Specific CD8⁺ T-cell Responses.

Cancer research

Cousin N,Cap S,Dihr M,Tacconi C,Detmar M,Dieterich LC

48-5698-82 was used in Immunocytochemistry to present a new lymphatic-specific PD-L1 knockout mouse model reveals that lymphatic endothelial PD-L1 expression reduces tumor immunity, inducing apoptosis in tumor-specific CD8+ central memory cells in tumor-draining lymph nodes.

Sun Aug 01 00:00:00 EDT 2021

Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.

Nature communications

Harsha Krovi S,Zhang J,Michaels-Foster MJ,Brunetti T,Loh L,Scott-Browne J,Gapin L

48-5698 was used in Flow cytometry/Cell sorting to highlight the common requirements for the development of innate-like T cells with a focus on how Hivep3 impacts the maturation of these lymphocytes.

Mon Dec 07 00:00:00 EST 2020

Coordinated Viral Control by Cytotoxic Lymphocytes Ensures Optimal Adaptive NK Cell Responses.

Cell reports

Diaz-Salazar C,Sun JC

48-5698 was used in Flow cytometry/Cell sorting to support a mechanism whereby cytotoxic innate and adaptive lymphocytes cooperate to ensure viral clearance and the establishment of robust clonal NK cell responses.

Tue Sep 22 00:00:00 EDT 2020

Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation.

Journal for immunotherapy of cancer

Wu B,Chiang HC,Sun X,Yuan B,Mitra P,Hu Y,Curiel TJ,Li R

48-5698 was used in Flow cytometry/Cell sorting to uncover a previously unrecognised role of adipocyte PD-L1 in mitigating obesity-related inflammation and metabolic dysfunction.

Sat Aug 01 00:00:00 EDT 2020

TLR9 Sensing of Self-DNA Controls Cell-Mediated Immunity to Listeria Infection via Rapid Conversion of Conventional CD4⁺ T Cells to T_reg.

Cell reports

Dolina JS,Lee J,Griswold RQ,Labarta-Bajo L,Kannan S,Greenbaum JA,Bahia El Idrissi N,Pont MJ,Croft M,Schoenberger SP

48-5698 was used in Flow cytometry/Cell sorting to resolve long-standing questions regarding the conditional requirement for TH amongst pathogens and reveal a remarkable degree of plasticity in the function of CD4+ T cells, which can be quickly converted to Tregin vivo by infection-mediated immune modulation.

Tue Apr 07 00:00:00 EDT 2020

Targeting ANXA1 abrogates Treg-mediated immune suppression in triple-negative breast cancer.

Journal for immunotherapy of cancer

Bai F,Zhang P,Fu Y,Chen H,Zhang M,Huang Q,Li D,Li B,Wu K

48-5698 was used in Flow cytometry/Cell sorting to show that ANXA1 expression is associated with lower survival and a higher risk of breast malignancy.

Wed Apr 01 00:00:00 EDT 2020

MicroRNA-127-3p controls murine hematopoietic stem cell maintenance by limiting differentiation.

Haematologica

Crisafulli L,Muggeo S,Uva P,Wang Y,Iwasaki M,Locatelli S,Anselmo A,Colombo FS,Carlo-Stella C,Cleary ML,Villa A,Gentner B,Ficara F

48-5698 was used in Flow cytometry/Cell sorting to investigate the contribution of micro-RNA in the regulation of self-renewal vs differentiation.

Sun Sep 01 00:00:00 EDT 2019

Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer.

Cell reports

Choi H,Deng J,Li S,Silk T,Dong L,Brea EJ,Houghton S,Redmond D,Zhong H,Boiarsky J,Akbay EA,Smith PD,Merghoub T,Wong KK,Wolchok JD

48-5698 was used in Flow cytometry/Cell sorting to set the foundation and show the importance of a combinatorial therapeutic strategy using pulsatile targeted therapy together with immunotherapy to optimally enhance tumor delay and promote long-term anti-tumor immunity.

Tue Apr 16 00:00:00 EDT 2019

Paracrine Activin-A Signaling Promotes Melanoma Growth and Metastasis through Immune Evasion.

The Journal of investigative dermatology

Donovan P,Dubey OA,Kallioinen S,Rogers KW,Muehlethaler K,Müller P,Rimoldi D,Constam DB

48-5698 was used in Immunocytochemistry to test whether a potential tumorigenic role for Activin signaling in melanoma may be curtailed in xenografts by the absence of a functional immune system.

Fri Dec 01 00:00:00 EST 2017

Resident macrophages of pancreatic islets have a seminal role in the initiation of autoimmune diabetes of NOD mice.

Proceedings of the National Academy of Sciences of the United States of America

Carrero JA,McCarthy DP,Ferris ST,Wan X,Hu H,Zinselmeyer BH,Vomund AN,Unanue ER

48-5698 was used in Flow cytometry/Cell sorting to study the role of resident macrophages of the pancreatic islets in the initiation of autoimmune diabetes of NOD mice.

Tue Nov 28 00:00:00 EST 2017

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Cell

Wang S,Xia P,Chen Y,Qu Y,Xiong Z,Ye B,Du Y,Tian Y,Yin Z,Xu Z,Fan Z

48-5698 was used in Flow cytometry/Cell sorting to identify a regulatory subpopulation of innate lymphoid cells that exists in the gut, and harbour a unique gene identity.

Thu Sep 21 00:00:00 EDT 2017

Intestinal Batf3-dependent dendritic cells are required for optimal antiviral T-cell responses in adult and neonatal mice.

Mucosal immunology

Sun T,Rojas OL,Li C,Ward LA,Philpott DJ,Gommerman JL

48-5698 was used in Flow cytometry/Cell sorting to study the dendritic cell subset(s) that provoke antiviral responses within the gut.

Mon May 01 00:00:00 EDT 2017

A Human Trypanosome Suppresses CD8+ T Cell Priming by Dendritic Cells through the Induction of Immune Regulatory CD4+ Foxp3+ T Cells.

PLoS pathogens

Ersching J,Basso AS,Kalich VL,Bortoluci KR,Rodrigues MM

48-5698 was used in Flow cytometry/Cell sorting to demonstrate a newly described mechanism of CD8+ T cell priming by CD4+ Foxp3+ T cells during Trypanosoma cruzi infection.

Wed Jun 01 00:00:00 EDT 2016

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

48-5698 was used in Flow cytometry/Cell sorting to show in mice, that maternal hepatitis B virus (HBV) impairs CD8(+) T cell responses to HBV in her offspring, resulting in HBV persistence.

Tue May 17 00:00:00 EDT 2016

Targeting myeloid-derived suppressor cells with colony stimulating factor-1 receptor blockade can reverse immune resistance to immunotherapy in indoleamine 2,3-dioxygenase-expressing tumors.

EBioMedicine

Holmgaard RB,Zamarin D,Lesokhin A,Merghoub T,Wolchok JD

48-5698 was used in Flow cytometry/Cell sorting to study the invovlement of myeloid-derived suppressor cells in IDO-expressing B16 tumour progression and therapy resistance.

Fri Apr 01 00:00:00 EDT 2016

Lineage-specific enhancers activate self-renewal genes in macrophages and embryonic stem cells.

Science (New York, N.Y.)

Soucie EL,Weng Z,Geirsdóttir L,Molawi K,Maurizio J,Fenouil R,Mossadegh-Keller N,Gimenez G,VanHille L,Beniazza M,Favret J,Berruyer C,Perrin P,Hacohen N,Andrau JC,Ferrier P,Dubreuil P,Sidow A,Sieweke MH

48-5698 was used in Flow cytometry/Cell sorting to investigate the mechanisms that accomplish self-renewal of differentiated macrophages.

Fri Feb 12 00:00:00 EST 2016

Myeloid cells expressing VEGF and arginase-1 following uptake of damaged retinal pigment epithelium suggests potential mechanism that drives the onset of choroidal angiogenesis in mice.

PloS one

Liu J,Copland DA,Horie S,Wu WK,Chen M,Xu Y,Paul Morgan B,Mack M,Xu H,Nicholson LB,Dick AD

Thu Jan 15 00:00:00 EST 2015

Beta-Chemokine CCL15 Affects the Adhesion and Migration of Hematopoietic Progenitor Cells.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

Richter R,Rüster B,Bistrian R,Forssmann WG,Seifried E,Henschler R

48-5698 was used in Flow cytometry/Cell sorting to point to a regulation of HPCs by CCL15 and ultimately improve HPC short-term engraftment in stem cell transplantation.

Thu Jan 01 00:00:00 EST 2015

Autophagy is a critical regulator of memory CD8(+) T cell formation.

eLife

Puleston DJ,Zhang H,Powell TJ,Lipina E,Sims S,Panse I,Watson AS,Cerundolo V,Townsend AR,Klenerman P,Simon AK

48-5698 was used in Flow cytometry/Cell sorting to show that mice lacking the autophagy gene Atg7 in T cells failed to establish CD8(+) T cell memory to influenza and MCMV infection.

Tue Nov 11 00:00:00 EST 2014

Dynamic changes in macrophage activation and proliferation during the development and resolution of intestinal inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Little MC,Hurst RJ,Else KJ

48-5698 was used in Flow cytometry/Cell sorting to study intestinal inflammation and the dynamic changes in macrophage activation and proliferation that occur during this process.

Sat Nov 01 00:00:00 EDT 2014

E and Id proteins influence invariant NKT cell sublineage differentiation and proliferation.

Journal of immunology (Baltimore, Md. : 1950)

D'Cruz LM,Stradner MH,Yang CY,Goldrath AW

48-5698 was used in Flow cytometry/Cell sorting to investigate the mechanism by which E and Id proteins control the development of invariant NKT sublineages after positive selection.

Sat Mar 01 00:00:00 EST 2014

Primate B-1 cells generate antigen-specific B cell responses to T cell-independent type 2 antigens.

Journal of immunology (Baltimore, Md. : 1950)

Yammani RD,Haas KM

Mon Apr 01 00:00:00 EDT 2013

IL-1R signaling in dendritic cells replaces pattern-recognition receptors in promoting CD8⁺ T cell responses to influenza A virus.

Nature immunology

Pang IK,Ichinohe T,Iwasaki A

Fri Mar 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; unnamed protein product

Gene Aliases: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

UniProt ID: (Human) P46013, (Mouse) E9PVX6

Entrez Gene ID: (Dog) 100686578, (Human) 4288, (Cynomolgus monkey) 102135895, (Rat) 291234, (Mouse) 17345

Function(s)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

Process(es)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding molecular_function