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Western blot analysis of Ki67 using a Ki67 monoclonal antibody (Product # MA5-15484) against a truncated Trx-Ki67 recombinant protein (1), truncated Ki67 (aa3118-3256)-His recombinant protein (2) and truncated Ki67 (aa3118-3256) human IgG Fc transfected CHO-K1 cell lysate (3).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of Ki167 (aa3118-3256) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15484 targets Ki67 in WB applications and shows reactivity with Human samples.
The MA5-15484 immunogen is purified recombinant fragment of Ki167 (aa3118-3256) expressed in E. Coli.
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
antigen identified by monoclonal antibody Ki-67; Ki-67; KIA; MKI67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105
KIA; MIB-; MIB-1; MKI67; PPP1R105