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|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from the human Ki-67 antigen.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||10-20 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21520 detects Ki67 in human, mouse, and rat samples.
PA1-21520 has been successfully used in immunohistochemistry (paraffin) and ICC/IF procedures. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH6.0, for 10-20 minutes, followed by coolong at room temperature for 20 minutes.
The PA1-21520 immunogen is a synthetic peptide from the human Ki-67 antigen. PA1-21520 recognizes an epiptope in the middle of the Ki67 protein.
Ki-67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumours is strong.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Tumor bioengineering using a transglutaminase crosslinked hydrogel.
PA1-21520 was used in immunocytochemistry to develop a 3D culturing system to mimic the tumor microenvironment using transglutaminase-cross-linked gelatin gel (Col-Tgel)
|Fang JY,Tan SJ,Yang Z,Tayag C,Han B||PloS one (9:null)||2014|
Minnelide reduces tumor burden in preclinical models of osteosarcoma.
PA1-21520 was used in immunohistochemistry to study the beneficial effects of minnelide on tumor burden and lung metastasis in osteosarcoma models
|Banerjee S,Thayanithy V,Sangwan V,Mackenzie TN,Saluja AK,Subramanian S||Cancer letters (335:412)||2013|
antigen identified by monoclonal antibody Ki-67; antigen KI-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105
KIA; MIB-; MIB-1; MKI67; PPP1R105