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|Tested species reactivity||Bovine, Dog, Deer, Horse, Hamster, Human, Mouse, Primate, Sheep, Pig, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Ki67.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||1 µg/mL|
|Immunohistochemistry (Frozen) (IHC (F))||1:100 - 1:1000|
|Immunohistochemistry - Free Floating (IHC (Free))||1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||0.1 - 5 µg/mL|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
OKN-007 decreases tumor necrosis and tumor cell proliferation and increases apoptosis in a preclinical F98 rat glioma model.
PA5-19462 was used in immunohistochemistry to study the regional effects of OKN-007 in the necrotic core and non-necrotic parenchyma of glioblastoma
|de Souza PC,Balasubramanian K,Njoku C,Smith N,Gillespie DL,Schwager A,Abdullah O,Ritchey JW,Fung KM,Saunders D,Jensen RL,Towner RA||Journal of magnetic resonance imaging : JMRI (42:1582)||2015|
Novel In Vivo model for combinatorial fluorescence labeling in mouse prostate.
PA5-19462 was used in immunohistochemistry to discuss animal models of prostate cancer
|Fang X,Gyabaah K,Nickkholgh B,Cline JM,Balaji KC||The Prostate (75:988)||2015|
Functional and mechanistic exploration of an adult neurogenesis-promoting small molecule.
PA5-19462 was used in immunohistochemistry to study the mechanism by which a synthetic small molecule enhances neurogenesis and hippocampal function in adult animals
|Petrik D,Jiang Y,Birnbaum SG,Powell CM,Kim MS,Hsieh J,Eisch AJ||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (26:3148)||2012|
β-catenin/cyclin D1 mediated development of suture mesenchyme in calvarial morphogenesis.
PA5-19462 was used in immunohistochemistry to investigate the role of beta-catenin/cyclin D1 during calvarial morphogenesis
|Mirando AJ,Maruyama T,Fu J,Yu HM,Hsu W||BMC developmental biology (10:null)||2010|
Genetic disruption of Smad7 impairs skeletal muscle growth and regeneration.
PA5-19462 was used in immunohistochemistry - frozen section to elucidate the role of SMAD7 in muscle
|Cohen TV,Kollias HD,Liu N,Ward CW,Wagner KR||The Journal of physiology (593:2479)||2015|
Darpp-32 and t-Darpp are differentially expressed in normal and malignant mouse mammary tissue.
PA5-19462 was used in immunohistochemistry - paraffin section to discuss the contributions of Darpp-32 and t-Darpp to the development of breast cancer.
|Christenson JL,Kane SE||Molecular cancer (13:null)||2014|
antigen identified by monoclonal antibody Ki-67; antigen KI-67; MKI67 FHA domain-interacting nucleolar phosphoprotein; nucleolar protein interacting with the FHA domain of MKI67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2, KIA, antigen KI-67
D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105