|Tested species reactivity||Bovine, Dog, Deer, Horse, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rat|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Ki67.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||1 µg/mL|
|Immunohistochemistry (Frozen) (IHC (F))||1:100 - 1:1000|
|Immunohistochemistry - Free Floating (IHC (Free))||1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||0.1 - 5 µg/mL|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Caspr Controls the Temporal Specification of Neural Progenitor Cells through Notch Signaling in the Developing Mouse Cerebral Cortex.
PA5-19462 was used in immunohistochemistry to report that Caspr regulates the temporal specification of cell fate in radial glial cells of the developing cerebral cortex through Notch signaling
|Wu ZQ,Li D,Huang Y,Chen XP,Huang W,Liu CF,Zhao HQ,Xu RX,Cheng M,Schachner M,Ma QH||Cerebral cortex (New York, N.Y. : 1991) (27:1369)||2017|
Molecular targeting of protein arginine deiminases to suppress colitis and prevent colon cancer.
PA5-19462 was used in immunohistochemistry to assess the effects of Chlor-amidine on miR-16
|Witalison EE,Cui X,Causey CP,Thompson PR,Hofseth LJ||Oncotarget (6:36053)||2015|
Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy.
PA5-19462 was used in immunohistochemistry - paraffin section to determine the role of autophagy in the regulation of liver regeneration after partial hepatectomy
|Lin CW,Chen YS,Lin CC,Chen YJ,Lo GH,Lee PH,Kuo PL,Dai CY,Huang JF,Chung WL,Yu ML||Scientific reports (5:null)||2015|
Inhibition of Pediatric Glioblastoma Tumor Growth by the Anti-Cancer Agent OKN-007 in Orthotopic Mouse Xenografts.
PA5-19462 was used in immunohistochemistry to characterize an orthotopic xenograft mouse model and use this model to assess the effects of an anti-cancer agent
|Coutinho de Souza P,Mallory S,Smith N,Saunders D,Li XN,McNall-Knapp RY,Fung KM,Towner RA||PloS one (10:null)||2015|
High fat diet increases melanoma cell growth in the bone marrow by inducing osteopontin and interleukin 6.
PA5-19462 was used in immunohistochemistry - paraffin section to study an increasing melanoma cell growth in the bone marrow by a high fat diet that induces osteopontin and interleukin 6
|Chen GL,Luo Y,Eriksson D,Meng X,Qian C,Bäuerle T,Chen XX,Schett G,Bozec A||Oncotarget (7:26653)||2016|
|Not Applicable||Not Cited||
Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex.
PA5-19462 was used in immunohistochemistry - paraffin section to investigate if and how mTOR regulates YAP
|Liang N,Zhang C,Dill P,Panasyuk G,Pion D,Koka V,Gallazzini M,Olson EN,Lam H,Henske EP,Dong Z,Apte U,Pallet N,Johnson RL,Terzi F,Kwiatkowski DJ,Scoazec JY,Martignoni G,Pende M||The Journal of experimental medicine (211:2249)||2014|
Darpp-32 and t-Darpp are differentially expressed in normal and malignant mouse mammary tissue.
PA5-19462 was used in immunohistochemistry - paraffin section to discuss the contributions of Darpp-32 and t-Darpp to the development of breast cancer.
|Christenson JL,Kane SE||Molecular cancer (13:null)||2014|
AKT1E¹¿K Is Oncogenic in Mouse Lung and Cooperates with Chemical Carcinogens in Inducing Lung Cancer.
PA5-19462 was used in immunohistochemistry to study the fact that AKT1E17K is oncogenic in mouse lung and cooperates with carcinogens in creating lung canc er
|Malanga D,Belmonte S,Colelli F,Scarfò M,De Marco C,Oliveira DM,Mirante T,Camastra C,Gagliardi M,Rizzuto A,Mignogna C,Paciello O,Papparella S,Fagman H,Viglietto G||PloS one (11:null)||2016|
OKN-007 decreases tumor necrosis and tumor cell proliferation and increases apoptosis in a preclinical F98 rat glioma model.
PA5-19462 was used in immunohistochemistry to study the regional effects of OKN-007 in the necrotic core and non-necrotic parenchyma of glioblastoma
|de Souza PC,Balasubramanian K,Njoku C,Smith N,Gillespie DL,Schwager A,Abdullah O,Ritchey JW,Fung KM,Saunders D,Jensen RL,Towner RA||Journal of magnetic resonance imaging : JMRI (42:1582)||2015|
Novel In Vivo model for combinatorial fluorescence labeling in mouse prostate.
PA5-19462 was used in immunohistochemistry to discuss animal models of prostate cancer
|Fang X,Gyabaah K,Nickkholgh B,Cline JM,Balaji KC||The Prostate (75:988)||2015|
Functional and mechanistic exploration of an adult neurogenesis-promoting small molecule.
PA5-19462 was used in immunohistochemistry to study the mechanism by which a synthetic small molecule enhances neurogenesis and hippocampal function in adult animals
|Petrik D,Jiang Y,Birnbaum SG,Powell CM,Kim MS,Hsieh J,Eisch AJ||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (26:3148)||2012|
ß-catenin/cyclin D1 mediated development of suture mesenchyme in calvarial morphogenesis.
PA5-19462 was used in immunohistochemistry to investigate the role of beta-catenin/cyclin D1 during calvarial morphogenesis
|Mirando AJ,Maruyama T,Fu J,Yu HM,Hsu W||BMC developmental biology (10:null)||2010|
Genetic disruption of Smad7 impairs skeletal muscle growth and regeneration.
PA5-19462 was used in immunohistochemistry - frozen section to elucidate the role of SMAD7 in muscle
|Cohen TV,Kollias HD,Liu N,Ward CW,Wagner KR||The Journal of physiology (593:2479)||2015|
Development and application of specific cytokine assays in tissue samples from a bottlenose dolphin with hyperinsulinemia.
PA5-19462 was used in immunohistochemistry - frozen section to characterize a dolphin with insulin resistance and fatty liver disease
|Eberle KC,Waters TE,Jensen ED,Venn-Watson SK,Sacco RE||Frontiers in endocrinology (4:null)||2013|