|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: M(1) V T S L N E D N E S(11) C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-640 detects kinesin 2 (KIF2) from mouse tissues.
PA1-640 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~95 kDa protein representing KIF2 from mouse brain homogenate.
PA1-640 immunizing peptide corresonds to amino acid residues 1-11 from mouse KIF2. This sequence is completely conserved in human KIF2. PA1-640 immunizing peptide (Cat. # PEP-040) is available for use in neutralization and control experiments.
Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure which includes a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. An expansive phylogenetic tree of kinesins and kinesin-related proteins (KRPs) has been assembled based on this information.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.