|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: Q(421) N Q V Q S L P A G S A E P(434)|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4332 detects Kos1 (a.k.a. TNK1) from mouse samples.
PA1-4332 has been successfully used in Western blot procedures.
PA1-4332 immunizing peptide corresponds to amino acid residues 421-434 from mouse Kos1 Protein.
Kinase of embryonic stem cells (Kos1) is a nonreceptor protein tyrosine kinase (NRPTK) and a new member of the Ack family of NRPTKs. Kos1 is a ubiquitously expressed 47-kDa protein with autotyrosine kinase activity that is developmentally regulated during embryogenesis. Kos1 is also upregulated following IL3 withdrawal from factor-dependent murine NSF/N1.H7 cells that undergo apoptosis, suggesting a role in growth inhibition. Data support a negative regulatory role for Kos1 in regulating the Ras-Raf1-MAPK growth pathway by a mechanism that requires its autotyrosine kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.