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Immunofluorescent analysis of Ku (green) in HeLa cells either left untreated (left panel) or treated with 50uM etoposide (right panel) for 3 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Ku (p70) monoclonal antibody (Product # MA5-13110) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Hamster, Human, Mouse, Non-human primate, Xenopus|
|Published species reactivity||Rat, Hamster, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Human placental extract designated as PSE1-PL|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.25 ug/test|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||0.25-0.5 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13110 targets Ku (p70) in immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, FACS and Western blot applications and shows reactivity with Human, Non-human primate, and Xenopus laevis samples. Reacts with mouse and hamster in Western blot applications only.
The MA5-13110 immunogen is human placental extract designated as PSE1-PL.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
C/EBPβ (CCAAT/enhancer-binding protein β) mediates progesterone production through transcriptional regulation in co-operation with SF-1 (steroidogenic factor-1).
MA5-13110 was used in western blot to study the role of cooperation between C/EBPbeta and steroidogenic factor-1 in the regulation of progesterone synthesis
|Mizutani T,Ju Y,Imamichi Y,Osaki T,Yazawa T,Kawabe S,Ishikane S,Matsumura T,Kanno M,Kamiki Y,Kimura K,Minamino N,Miyamoto K||The Biochemical journal (460:459)||2014|
DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1.
MA5-13110 was used in immunocytochemistry and western blot to study the role of DNA-PK and PARP in the mechanism by which DNA damage inhibits the synthesis of rRNA
|Calkins AS,Iglehart JD,Lazaro JB||Nucleic acids research (41:7378)||2013|
A noncatalytic function of the ligation complex during nonhomologous end joining.
MA5-13110 was used in western blot to study the significance of non-catalytic functions of DNA Ligase IV in the ligation complex during non-homologous end-joining
|Cottarel J,Frit P,Bombarde O,Salles B,Négrel A,Bernard S,Jeggo PA,Lieber MR,Modesti M,Calsou P||The Journal of cell biology (200:173)||2013|
Novel Insights into the Molecular Mechanism of Action of DNA Hypomethylating Agents: Role of Protein Kinase C δ in Decitabine-Induced Degradation of DNA Methyltransferase 1.
MA5-13110 was used in western blot to study the role of PKCdelta in the decitabine-induced degradation of the maintenance methyltransferase DNMT1
|Datta J,Ghoshal K,Motiwala T,Jacob ST||Genes & cancer (3:71)||2012|
Ku proteins function as corepressors to regulate farnesoid X receptor-mediated gene expression.
MA5-13110 was used in western blot to investigate the effect of Ku proteins on farnesoid X receptor-mediated gene expression
|Ohno M,Kunimoto M,Nishizuka M,Osada S,Imagawa M||Biochemical and biophysical research communications (390:738)||2009|
Involvement of p54(nrb), a PSF partner protein, in DNA double-strand break repair and radioresistance.
MA5-13110 was used in western blot to investigate the role of p54(nrb) in DNA double-strand break repair in vivo
|Li S,Kuhne WW,Kulharya A,Hudson FZ,Ha K,Cao Z,Dynan WS||Nucleic acids research (37:6746)||2009|
Molecular analysis of Ku redox regulation.
MA5-13110 was used in western blot to investigate the regulatory effect of redox conditions on the function of Ku
|Bennett SM,Neher TM,Shatilla A,Turchi JJ||BMC molecular biology (10:null)||2009|
Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo.
MA5-13110 was used in western blot to investigate the effect of BRCA2 on the radio-sensitization of human tumor cells
|Yu D,Sekine E,Fujimori A,Ochiya T,Okayasu R||Cancer science (99:810)||2008|
Treatment of PC12 cells with nerve growth factor induces proteasomal degradation of T-cadherin that requires tyrosine phosphorylation of its cadherin domain.
MA5-13110 was used in western blot to investigate the mechanism of T-cadherin degradation in PC-12 cells treated with nerve growth factor
|Bai S,Datta J,Jacob ST,Ghoshal K||The Journal of biological chemistry (282:27171)||2007|
Identification of T-cadherin as a novel target of DNA methyltransferase 3B and its role in the suppression of nerve growth factor-mediated neurite outgrowth in PC12 cells.
MA5-13110 was used in western blot to study the role of T-cadherin in the nerve growth factor-mediated neurite outgrowth
|Bai S,Ghoshal K,Jacob ST||The Journal of biological chemistry (281:13604)||2006|
Development of new EBV-based vectors for stable expression of small interfering RNA to mimick human syndromes: application to NER gene silencing.
MA5-13110 was used in western blot to investigate a novel method of stably expressing small interfering RNA to generate human disease models
|Biard DS,Despras E,Sarasin A,Angulo JF||Molecular cancer research : MCR (3:519)||2005|
DNA-dependent protein kinase and XRCC4-DNA ligase IV mobilization in the cell in response to DNA double strand breaks.
MA5-13110 was used in western blot to study the mobilization of DNA-dependent protein kinase and XRCC4-ligase IV in response to DNA double strand breaks
|Drouet J,Delteil C,Lefrançois J,Concannon P,Salles B,Calsou P||The Journal of biological chemistry (280:7060)||2005|
Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining.
MA5-13110 was used in western blot to study an alternative pathway for the repair of DNA double-strand breaks that involves poly(ADP-ribose) polymerase-1 and a XRCC1-DNA ligase III complex
|Audebert M,Salles B,Calsou P||The Journal of biological chemistry (279:55117)||2004|
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.
MA5-13110 was used in western blot to study two biochemically distinct pathways of non-homologous end joining that are differentially regulated by DNA-PK
|Udayakumar D,Bladen CL,Hudson FZ,Dynan WS||The Journal of biological chemistry (278:41631)||2003|
Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment.
MA5-13110 was used in western blot to investigate the relationship and assembly of specific proteins during DNA repair process
|Calsou P,Delteil C,Frit P,Drouet J,Salles B||Journal of molecular biology (326:93)||2003|
Evidence implicating Ku antigen as a structural factor in RNA polymerase II-mediated transcription.
MA5-13110 was used in western blot to study the potential role of the Ku antigen within the RNA polymerase II holoenzyme
|Bertinato J,Tomlinson JJ,Schild-Poulter C,Haché RJ||Gene (302:53)||2003|
Role of de novo DNA methyltransferases and methyl CpG-binding proteins in gene silencing in a rat hepatoma.
MA5-13110 was used in western blot to investigate the involvement of the DNA methylation machinery in gene silencing in rat hepatoma and liver
|Majumder S,Ghoshal K,Datta J,Bai S,Dong X,Quan N,Plass C,Jacob ST||The Journal of biological chemistry (277:16048)||2002|
Expression and chromosome location of hamster Ku70 and Ku80.
MA5-13110 was used in western blot to study the chromosomal location of hamster Ku70 and Ku80 and the effect of ionizing radiation on their expression levels
|Koike M,Kuroiwa A,Koike A,Shiomi T,Matsuda Y||Cytogenetics and cell genetics (93:52)||2001|
Ku entry into DNA inhibits inward DNA transactions in vitro.
MA5-13110 was used in western blot to study the effect of Ku entry into DNA on local DNA processes and the regulatory role of DNA-dependent protein kinase
|Frit P,Li RY,Arzel D,Salles B,Calsou P||The Journal of biological chemistry (275:35684)||2000|
Poly(ADP-ribose) polymerase and Ku autoantigen form a complex and synergistically bind to matrix attachment sequences.
MA5-13110 was used in western blot to study the binding of poly(ADP-ribose) polymerase and Ku autoantigen to matrix attachment sequences
|Galande S,Kohwi-Shigematsu T||The Journal of biological chemistry (274:20521)||1999|
Human RTEL1 deficiency causes Hoyeraal-Hreidarsson syndrome with short telomeres and genome instability.
MA5-13110 was used in immunocytochemistry and western blot to study the role of RTEL1 deficiency as a novel cause of Hoyerdaal-Hreidarssen syndrome
|Le Guen T,Jullien L,Touzot F,Schertzer M,Gaillard L,Perderiset M,Carpentier W,Nitschke P,Picard C,Couillault G,Soulier J,Fischer A,Callebaut I,Jabado N,Londono-Vallejo A,de Villartay JP,Revy P||Human molecular genetics (22:3239)||2013|
Rad54B targeting to DNA double-strand break repair sites requires complex formation with S100A11.
MA5-13110 was used in immunocytochemistry to examine the interaction between Rad54B and S100A11 and its role in DNA damage repair
|Murzik U,Hemmerich P,Weidtkamp-Peters S,Ulbricht T,Bussen W,Hentschel J,von Eggeling F,Melle C||Molecular biology of the cell (19:2926)||2008|
Telomere dysfunction-induced foci arise with the onset of telomeric deletions and complex chromosomal aberrations in resistant chronic lymphocytic leukemia cells.
MA5-13110 was used in ChIP assay to investigate the effect of telomere dysfunction-mediated chromosomal aberration on the prognosis of chronic lymphocytic leukemia patients
|Brugat T,Nguyen-Khac F,Grelier A,Merle-Béral H,Delic J||Blood (116:239)||2010|
5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 5'-dRP/AP lyase Ku70; 70 kDa subunit; 70 kDa subunit of Ku antigen; 70kDa; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II; ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Ku autoantigen; Ku autoantigen p70 subunit; ku autoantigen protein p70 homolog; Ku autoantigen, 70kDa; Ku p70; lupus Ku autoantigen protein p70; thyroid; thyroid autoantigen 70 kDa; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); thyroid-lupus autoantigen p70; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 6
70kDa; CTC75; CTCBF; G22P1; KU70; ML8; TLAA; XRCC6