Immunofluorescent analysis of Ku70+Ku80 (green) showing staining in the nucleus of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Ku70+Ku80 monoclonal antibody (Product # MA1-21818) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat, Xenopus|
|Published species reactivity||Non-human primate, Hamster, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human B cell nuclei from plasmacytoid 2p68 cells.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-1ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/mg of protein lysate|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21818 detects Ku70/Ku80 in human, non-human primate, mouse, rat and xenopus laevis samples. It reacts with a conformational epitope of the p70/p80 dimer, which is destroyed during Western blot. This antibody is not recommended for Western blot procedures and will not cross-react with bovine or rabbit.
The Ku autoantigen is a heterodimer of 70 kDa (p70) and ~80 kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The DNA damage/repair cascade in glioblastoma cell lines after chemotherapeutic agent treatment.
MA1-21818 was used in immunocytochemistry, immunohistochemistry - paraffin section, and western blot to analyze chemotherapeutic agent treatment and the DNA damage/repair cascade in glioblastoma cell lines
|Annovazzi L,Caldera V,Mellai M,Riganti C,Battaglia L,Chirio D,Melcarne A,Schiffer D||International journal of oncology (46:2299)||2015|
Accumulation of Ku80 proteins at DNA double-strand breaks in living cells.
MA1-21818 was used in immunocytochemistry to study the accumulation Ku80 proteins at DNA double-strand breaks in living cells
|Koike M,Koike A||Experimental cell research (314:1061)||2008|
Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.
MA1-21818 was used in EMSA to study the prognostic value of estrogen resonse element binding proteins in breast cancer
|Kruer TL,Cummins TD,Powell DW,Wittliff JL||Clinical biochemistry (46:1739)||2013|
Loading of the nonhomologous end joining factor, Ku, on protein-occluded DNA ends.
MA1-21818 was used in EMSA to study the mechanism by which the nonhomologous end joining factor Ku is loaded on protein-occluded DNA ends
|Roberts SA,Ramsden DA||The Journal of biological chemistry (282:10605)||2007|
Ongoing immunoglobulin class switch DNA recombination in lupus B cells: analysis of switch regulatory regions.
MA1-21818 was used in EMSA to study the role of exogenous signals in ongoing immunoglobulin class switching DNA recombination in lupus B cells
|Liu S,Cerutti A,Casali P,Crow MK||Autoimmunity (37:431)||2004|
Differential DNA binding of Ku antigen determines its involvement in DNA replication.
MA1-21818 was used in EMSA to study the role of differential DNA binding of Ku antigen in determining its involvement in DNA replication
|Schild-Poulter C,Matheos D,Novac O,Cui B,Giffin W,Ruiz MT,Price GB,Zannis-Hadjopoulos M,Haché RJ||DNA and cell biology (22:65)||2003|
The repressor which binds the -75 GATA motif of the GPB promoter contains Ku70 as the DNA binding subunit.
MA1-21818 was used in EMSA to study the role of the Ku70 protein in transcriptionally regulating the erythroid glycoprotein B promoter
|Camara-Clayette V,Thomas D,Rahuel C,Barbey R,Cartron JP,Bertrand O||Nucleic acids research (27:1656)||1999|
Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.
MA1-21818 was used in western blot to study the role of PARP1 and MRN proteins in the repair of DNA double strand breaks
|Cheng Q,Barboule N,Frit P,Gomez D,Bombarde O,Couderc B,Ren GS,Salles B,Calsou P||Nucleic acids research (39:9605)||2011|
Biochemical evidence for Ku-independent backup pathways of NHEJ.
MA1-21818 was used in western blot to study alternative backup pathways of non-homologous end joining that are independent of Ku
|Wang H,Perrault AR,Takeda Y,Qin W,Wang H,Iliakis G||Nucleic acids research (31:5377)||2003|
Physical interaction between epidermal growth factor receptor and DNA-dependent protein kinase in mammalian cells.
MA1-21818 was used in western blot to study the biological functions of the epidermal growth factor receptor and DNA-dependent protein kinase interaction
|Bandyopadhyay D,Mandal M,Adam L,Mendelsohn J,Kumar R||The Journal of biological chemistry (273:1568)||1998|
The DNA repair complex Ku70/86 modulates Apaf1 expression upon DNA damage.
MA1-21818 was used in ChIP assay to study the role of the Ku70/86 complex in modulating Apaf-1 expression upon DNA damage
|De Zio D,Bordi M,Tino E,Lanzuolo C,Ferraro E,Mora E,Ciccosanti F,Fimia GM,Orlando V,Cecconi F||Cell death and differentiation (18:516)||2011|
The effect of DNA-dependent protein kinase on adeno-associated virus replication.
MA1-21818 was used in immunoprecipitation to study the role of DNA-dependent protein kinase in adeno-associated virus replication
|Choi YK,Nash K,Byrne BJ,Muzyczka N,Song S||PloS one (5:null)||2010|
Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends.
MA1-21818 was used in immunoprecipitation to study the interaction of Artemis with proteins involved of the non-homologous end joining pathway
|Drouet J,Frit P,Delteil C,de Villartay JP,Salles B,Calsou P||The Journal of biological chemistry (281:27784)||2006|
Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining.
MA1-21818 was used in immunoprecipitation to study an alternative pathway for the repair of DNA double-strand breaks that involves poly(ADP-ribose) polymerase-1 and a XRCC1-DNA ligase III complex
|Audebert M,Salles B,Calsou P||The Journal of biological chemistry (279:55117)||2004|
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.
MA1-21818 was used in immunoprecipitation to study the potential anti-recombinogenic role of BLM DNA helicase in double-strand break processing
|Onclercq-Delic R,Calsou P,Delteil C,Salles B,Papadopoulo D,Amor-Guéret M||Nucleic acids research (31:6272)||2003|
|Non-human primate||Not Cited||
In vivo association of Ku with mammalian origins of DNA replication.
MA1-21818 was used in immunoprecipitation to investigate the role of Ku protein in the DNA replication in mammalia
|Novac O,Matheos D,Araujo FD,Price GB,Zannis-Hadjopoulos M||Molecular biology of the cell (12:3386)||2001|
Ku entry into DNA inhibits inward DNA transactions in vitro.
MA1-21818 was used in immunoprecipitation to study the effect of Ku entry into DNA on local DNA processes and the regulatory role of DNA-dependent protein kinase
|Frit P,Li RY,Arzel D,Salles B,Calsou P||The Journal of biological chemistry (275:35684)||2000|
Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.
MA1-21818 was used in immunoprecipitation to study the role of replication protein A in the replication-mediated DNA damage caused by the topoisomerase inhibitor camptothecin
|Shao RG,Cao CX,Zhang H,Kohn KW,Wold MS,Pommier Y||The EMBO journal (18:1397)||1999|
Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
MA1-21818 was used in blocking or activating experiment to study the role of Ku70/80 and DNA-PK in regulating RAG-mediated cleavage and the significance for the 12/23 rule specificity
|Sawchuk DJ,Mansilla-Soto J,Alarcon C,Singha NC,Langen H,Bianchi ME,Lees-Miller SP,Nussenzweig MC,Cortes P||The Journal of biological chemistry (279:29821)||2004|