Invitrogen

Ku70/Ku80 Monoclonal Antibody (162)

5 Published Figures

21 References

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Product Image Gallery

Ku70/Ku80 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Ku70+Ku80 (green) showing staining in the nucleus of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Ku70+Ku80 monoclonal antibody (Product # MA1-21818) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

Ku70/Ku80 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil stained using a Ku monoclonal antibody (Product # MA1-21818) with a peroxidase-conjugate and AEC chromogen. Note nuclear staining of the cells.

Ku70/Ku80 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of Ku70+Ku80 in C2C12 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Ku70+Ku80 monoclonal antibody (Product # MA1-21818) at a dilution of 1 µg/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Ku70/Ku80 Antibody in Western Blot (WB) — Figure 1 Rad52 deficiency increases CSR. ( a ) B cells purified from Rad52 +/+ and Rad52 -/- C57BL/6 littermates were stimulated with mCD154 or LPS plus IL-4 (for CSR to IgG1), LPS alone (IgG3), LPS plus IFN-gamma (IgG2c) or LPS plus TGF-beta, IL-4, IL-5 and anti-delta mAb/dex (IgA). Purified Aicda +/+ and Aicda -/- B cells were stimulated with LPS plus IL-4. After 96 h of culture, the cells were analysed for surface IgG1, IgG3, IgG2c or IgA by flow cytometry. ( b ) Proliferation of Rad52 +/+ and Rad52 -/- B cells labelled with CFSE and stimulated for 72 h with different amounts of LPS or mCD154 plus IL-4. Progressive left shift of fluorescence intensity indicates B220 + B cell division. ( c ) Proliferation of Rad52 +/+ and Rad52 -/- B cells labelled with the cell division tracking fluorochrome CFSE and stimulated by mCD154 plus IL-4 or LPS plus IL-4 for 96 h. CFSE intensity and surface IgG1 expression analysed by flow cytometry. Proportion of surface IgG1 + B cells at each cell division indicated. P -values determined using a paired Student's t -test. Data are from one representative (left panels of each condition) or mean+-s.d. of three independent experiments (right panels of each condition). ( d ) Recombinant Smu-Sgamma1 or Smu-Sgamma3 DNAs analysed by digestion-circularization PCR (DC-PCR) using serially twofold diluted HindIII digested and T4 DNA ligase-ligated genomic DNA from Rad52 +/+ or Rad52 -/- B cells after stimulation with LPS or LPS plus IL-4 for 96 h. Gap

Ku70/Ku80 Antibody in Immunoprecipitation (IP) — Published figure using Ku70/Ku80 monoclonal antibody (Product # MA1-21818) in Immunoprecipitation

Ku70/Ku80 Antibody in ChIP Assay (ChIP) — Published figure using Ku70/Ku80 monoclonal antibody (Product # MA1-21818) in ChIP Assay

Product Details

MA1-21818

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	1-2 µg/mL	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 2 publications 2 publications
Flow Cytometry (Flow)	0.5-1 µg/test	-
ChIP assay (ChIP)	-	View 2 publications 2 publications
Western Blot (WB)	-	View 4 publications 4 publications
Gel Shift (GS)	-	View 5 publications 5 publications
Immunoprecipitation (IP)	-	View 7 publications 7 publications
Neutralization (Neu)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate, Rat, Xenopus
Published species	Hamster, Human, Mouse, Non-human primate
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	162
Immunogen	Human B cell nuclei from plasmacytoid 2p68 cells.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Ammonium sulfate precipitation
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_561990

Product Specific Information

MA1-21818 detects Ku70/Ku80 in human, non-human primate, mouse, rat and xenopus laevis samples. It reacts with a conformational epitope of the p70/p80 dimer, which is destroyed during Western blot. This antibody is not recommended for Western blot procedures and will not cross-react with bovine or rabbit.

Target Information

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination.

Nature communications

Zan H,Tat C,Qiu Z,Taylor JR,Guerrero JA,Shen T,Casali P

MA121818 was used in ChIP assay and EMSA to assess the contributions of single-strand annealing factors HR Rad52 and translesion DNA polymerase q to CSR

Wed Feb 08 00:00:00 EST 2017

Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination.

Nature communications

Zan H,Tat C,Qiu Z,Taylor JR,Guerrero JA,Shen T,Casali P

MA121818 was used in ChIP assay and EMSA to assess the contributions of single-strand annealing factors HR Rad52 and translesion DNA polymerase q to CSR

Wed Feb 08 00:00:00 EST 2017

The DNA damage/repair cascade in glioblastoma cell lines after chemotherapeutic agent treatment.

International journal of oncology

Annovazzi L,Caldera V,Mellai M,Riganti C,Battaglia L,Chirio D,Melcarne A,Schiffer D

MA1-21818 was used in immunocytochemistry, immunohistochemistry - paraffin section, and western blot to analyze chemotherapeutic agent treatment and the DNA damage/repair cascade in glioblastoma cell lines

Tue Feb 02 00:00:00 EST 2016

Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.

Clinical biochemistry

Kruer TL,Cummins TD,Powell DW,Wittliff JL

MA1-21818 was used in EMSA to study the prognostic value of estrogen resonse element binding proteins in breast cancer

Fri Nov 01 00:00:00 EDT 2013

Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.

Nucleic acids research

Cheng Q,Barboule N,Frit P,Gomez D,Bombarde O,Couderc B,Ren GS,Salles B,Calsou P

MA1-21818 was used in western blot to study the role of PARP1 and MRN proteins in the repair of DNA double strand breaks

Thu Dec 01 00:00:00 EST 2011

The DNA repair complex Ku70/86 modulates Apaf1 expression upon DNA damage.

Cell death and differentiation

De Zio D,Bordi M,Tino E,Lanzuolo C,Ferraro E,Mora E,Ciccosanti F,Fimia GM,Orlando V,Cecconi F

MA1-21818 was used in ChIP assay to study the role of the Ku70/86 complex in modulating Apaf-1 expression upon DNA damage

Tue Mar 01 00:00:00 EST 2011

The effect of DNA-dependent protein kinase on adeno-associated virus replication.

PloS one

Choi YK,Nash K,Byrne BJ,Muzyczka N,Song S

MA1-21818 was used in immunoprecipitation to study the role of DNA-dependent protein kinase in adeno-associated virus replication

Mon Dec 20 00:00:00 EST 2010

Accumulation of Ku80 proteins at DNA double-strand breaks in living cells.

Experimental cell research

Koike M,Koike A

MA1-21818 was used in immunocytochemistry to study the accumulation Ku80 proteins at DNA double-strand breaks in living cells

Mon Mar 10 00:00:00 EDT 2008

Loading of the nonhomologous end joining factor, Ku, on protein-occluded DNA ends.

The Journal of biological chemistry

Roberts SA,Ramsden DA

MA1-21818 was used in EMSA to study the mechanism by which the nonhomologous end joining factor Ku is loaded on protein-occluded DNA ends

Fri Apr 06 00:00:00 EDT 2007

Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends.

The Journal of biological chemistry

Drouet J,Frit P,Delteil C,de Villartay JP,Salles B,Calsou P

MA1-21818 was used in immunoprecipitation to study the interaction of Artemis with proteins involved of the non-homologous end joining pathway

Fri Sep 22 00:00:00 EDT 2006

Ongoing immunoglobulin class switch DNA recombination in lupus B cells: analysis of switch regulatory regions.

Autoimmunity

Liu S,Cerutti A,Casali P,Crow MK

MA1-21818 was used in EMSA to study the role of exogenous signals in ongoing immunoglobulin class switching DNA recombination in lupus B cells

Thu May 12 00:00:00 EDT 2005

Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining.

The Journal of biological chemistry

Audebert M,Salles B,Calsou P

MA1-21818 was used in immunoprecipitation to study an alternative pathway for the repair of DNA double-strand breaks that involves poly(ADP-ribose) polymerase-1 and a XRCC1-DNA ligase III complex

Fri Dec 31 00:00:00 EST 2004

Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.

The Journal of biological chemistry

Sawchuk DJ,Mansilla-Soto J,Alarcon C,Singha NC,Langen H,Bianchi ME,Lees-Miller SP,Nussenzweig MC,Cortes P

MA1-21818 was used in blocking or activating experiment to study the role of Ku70/80 and DNA-PK in regulating RAG-mediated cleavage and the significance for the 12/23 rule specificity

Fri Jul 09 00:00:00 EDT 2004

Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.

Nucleic acids research

Onclercq-Delic R,Calsou P,Delteil C,Salles B,Papadopoulo D,Amor-Guéret M

MA1-21818 was used in immunoprecipitation to study the potential anti-recombinogenic role of BLM DNA helicase in double-strand break processing

Sat Nov 01 00:00:00 EST 2003

Biochemical evidence for Ku-independent backup pathways of NHEJ.

Nucleic acids research

Wang H,Perrault AR,Takeda Y,Qin W,Wang H,Iliakis G

MA1-21818 was used in western blot to study alternative backup pathways of non-homologous end joining that are independent of Ku

Mon Sep 15 00:00:00 EDT 2003

Differential DNA binding of Ku antigen determines its involvement in DNA replication.

DNA and cell biology

Schild-Poulter C,Matheos D,Novac O,Cui B,Giffin W,Ruiz MT,Price GB,Zannis-Hadjopoulos M,Haché RJ

MA1-21818 was used in EMSA to study the role of differential DNA binding of Ku antigen in determining its involvement in DNA replication

Sat Feb 01 00:00:00 EST 2003

In vivo association of Ku with mammalian origins of DNA replication.

Molecular biology of the cell

Novac O,Matheos D,Araujo FD,Price GB,Zannis-Hadjopoulos M

MA1-21818 was used in immunoprecipitation to investigate the role of Ku protein in the DNA replication in mammalia

Thu Nov 01 00:00:00 EST 2001

Ku entry into DNA inhibits inward DNA transactions in vitro.

The Journal of biological chemistry

Frit P,Li RY,Arzel D,Salles B,Calsou P

MA1-21818 was used in immunoprecipitation to study the effect of Ku entry into DNA on local DNA processes and the regulatory role of DNA-dependent protein kinase

Fri Nov 17 00:00:00 EST 2000

The repressor which binds the -75 GATA motif of the GPB promoter contains Ku70 as the DNA binding subunit.

Nucleic acids research

Camara-Clayette V,Thomas D,Rahuel C,Barbey R,Cartron JP,Bertrand O

MA1-21818 was used in EMSA to study the role of the Ku70 protein in transcriptionally regulating the erythroid glycoprotein B promoter

Thu Apr 01 00:00:00 EST 1999

Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.

The EMBO journal

Shao RG,Cao CX,Zhang H,Kohn KW,Wold MS,Pommier Y

MA1-21818 was used in immunoprecipitation to study the role of replication protein A in the replication-mediated DNA damage caused by the topoisomerase inhibitor camptothecin

Mon Mar 01 00:00:00 EST 1999

Physical interaction between epidermal growth factor receptor and DNA-dependent protein kinase in mammalian cells.

The Journal of biological chemistry

Bandyopadhyay D,Mandal M,Adam L,Mendelsohn J,Kumar R

MA1-21818 was used in western blot to study the biological functions of the epidermal growth factor receptor and DNA-dependent protein kinase interaction

Fri Jan 16 00:00:00 EST 1998

Bioinformatics

Protein Aliases: 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 5'-dRP/AP lyase Ku70; 70 kDa subunit of Ku antigen; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II 80 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC75; CTC85; CTCBF; DNA repair protein XRCC5; DNA repair protein XRCC6; Ku autoantigen p70 subunit; Ku autoantigen protein p70 homolog; Ku autoantigen protein p86 homolog; Ku autoantigen, 70kDa; Ku autoantigen, 80kD); Ku autoantigen, 80kDa; Ku p70; Ku p80; Ku70; Ku70 DNA-binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa); Ku80; Ku86; Ku86 autoantigen related protein 1; Lupus Ku autoantigen protein p70; Lupus Ku autoantigen protein p86; Nuclear factor IV; thyroid autoantigen; thyroid autoantigen 70 kDa; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); Thyroid-lupus autoantigen; thyroid-lupus autoantigen p70; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kDa); X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross complementation (double-strand-break rejoining; X-ray repair cross complementation (double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross-complementing protein 5; X-ray repair cross-complementing protein 6

Gene Aliases: 70kDa; AI314015; CTC75; CTCBF; G22P1; G22P2; KARP-1; KARP1; KU70; KU80; Ku86; KUB2; Kup70; Kup80; ML8; NFIV; TLAA; XELAEV_18023498mg; XELAEV_18044412mg; XRCC5; xrcc5-a; XRCC6

UniProt ID: (Human) P13010, (Human) P12956, (Mouse) P27641, (Mouse) P23475

Entrez Gene ID: (Human) 7520, (Human) 2547, (Rat) 363247, (Mouse) 22596, (Rat) 25019, (Mouse) 14375, (Xenopus) 394397, (Xenopus) 398357

Molecular Function: DNA helicase

Function(s)

telomere maintenance double-strand break repair double-strand break repair via nonhomologous end joining DNA recombination transcription, DNA-templated cell proliferation positive regulation of type I interferon production DNA duplex unwinding negative regulation of transcription, DNA-templated regulation of smooth muscle cell proliferation positive regulation of neurogenesis hematopoietic stem cell differentiation cellular response to gamma radiation cellular response to X-ray establishment of integrated proviral latency negative regulation of t-circle formation DNA ligation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter protein heterotetramerization double-strand break repair via classical nonhomologous end joining brain development response to drug cellular response to fatty acid cellular hyperosmotic salinity response DNA repair regulation of transcription, DNA-templated cellular response to DNA damage stimulus response to ionizing radiation V(D)J recombination metabolic process

Process(es)

DNA binding damaged DNA binding double-stranded DNA binding double-stranded telomeric DNA binding ATP-dependent DNA helicase activity protein binding ATP binding protein C-terminus binding ubiquitin protein ligase binding telomeric DNA binding transcription regulatory region DNA binding poly(A) RNA binding macromolecular complex binding 5'-deoxyribose-5-phosphate lyase activity nucleotide binding helicase activity hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity lyase activity

Ku70/Ku80 Monoclonal Antibody (162)

Product Details

MA1-21818

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Western Blot (WB)

Gel Shift (GS)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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