|Western Blot (WB)||1:100-1:500|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Hamster, Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat liver lysosomal membrane preparations|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
MA1-164 detects glycosylated LAMP-1 at ~130kD.
M-PER (Product # 78501), IP Lysis Buffer (Product # 87787), or a 1% Triton X-100 lysis buffer are recommended for Western blot. LAMP-1 was not detected when RIPA buffer was used to generate cell lysates.
Lysosome associated membrane proteins (LAMP1), also known as Igp 120 or IgpA, is a type 1 intergral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly invovled in the adhesion of PBMC to the site of inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 120 kDa lysosomal membrane glycoprotein; CD107 antigen-like family member A; CD107a; Lamp I; LAMP-1; LAMPA; LGP-120; LGP-A; LGP120; Lysosomal associated membrane protein 1 (120 kDa); lysosomal membrane glycoprotein 1; Lysosomal membrane glycoprotein A; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); Lysosome-associated membrane protein 1; P2B
Gene Aliases: AI196048; CD107a; I79_011073; LAMP-1; LAMP1; LAMPA; LGP-A; LGP120; LGPA
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