|Tested species reactivity||Dog, Human, Mink|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MDCK (Madin-Darby Canine Kidney) cells.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not react with mouse or rat.
Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). LAMP-2, a highly glycosylated protein associated with the lysosome, has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danonâ€™s disease is associated with the accumulation of autophagic material in striated myocytes. LAMP-2 exists in multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.