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Western blot analysis was performed on whole cell extracts (30 µg lysate) of C2C12 (Lane 1), NIH/3T3 (Lane 2) and PC-12 (Lane 3). The blot was probed with Anti-LAMP2 Rabbit Polyclonal Antibody (Product # 51-2200, 2 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A27036, 0.4µg/ml, 1:2500 dilution). A ~110 kDa band corresponding to LAMP2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by overnight transfer method. The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product# SLF2000S). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide of the cytosolic tail of rat LAMP-2A (GLKRHHTGYEQF)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Inhibition Assays (IA)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody was tested for specificity in rat and mouse (it does not cross-react with the human variant) and was demonstrated to be highly selective for LAMP-2A without cross-reacting with LAMP-2B or C.
This protein was previously known as lgp96.
Lysosome Associated Membrane Protein 2 (LAMP 2) is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. Mature, functional LAMP 2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of ~100-110 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. This protein has 3 variant forms: LAMP-2A, -2B, and 2C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Chaperone-Mediated Autophagy after Traumatic Brain Injury.
51-2200 was used in western blot to examine how chaperone-mediated autophagy and the ubiquitin-proteasomal system are regulated after traumatic brain injury.
|Park Y,Liu C,Luo T,Dietrich WD,Bramlett H,Hu B||Journal of neurotrauma (32:1449)||2015|
α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2.
51-2200 was used in western blot to investigate how endolysosomal dysfunction causes Parkinson's disease-related neurodegeneration
|Kett LR,Stiller B,Bernath MM,Tasset I,Blesa J,Jackson-Lewis V,Chan RB,Zhou B,Di Paolo G,Przedborski S,Cuervo AM,Dauer WT||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:5724)||2015|
PP2A blockade inhibits autophagy and causes intraneuronal accumulation of ubiquitinated proteins.
51-2200 was used in western blot to study the effect of protein phosphatase 2A modulation on neuronal autophagy.
|Magnaudeix A,Wilson CM,Page G,Bauvy C,Codogno P,Lévêque P,Labrousse F,Corre-Delage M,Yardin C,Terro F||Neurobiology of aging (34:770)||2013|
Amphetamine and methamphetamine reduce striatal dopamine transporter function without concurrent dopamine transporter relocalization.
51-2200 was used in western blot to determine dopamine transporter localization following in vivo amphetamine and methamphetamine administration.
|German CL,Hanson GR,Fleckenstein AE||Journal of neurochemistry (123:288)||2012|
Regional deficiencies in chaperone-mediated autophagy underlie α-synuclein aggregation and neurodegeneration.
51-2200 was used in western blot to investigate why certain parts of the brain are more vulnerable to protein aggregation.
|Malkus KA,Ischiropoulos H||Neurobiology of disease (46:732)||2012|
|Human||Not Cited||Epidermal growth factor receptor translocation to the mitochondria: regulation and effect.||Demory ML,Boerner JL,Davidson R,Faust W,Miyake T,Lee I,Hüttemann M,Douglas R,Haddad G,Parsons SJ||The Journal of biological chemistry (284:36592)||2009|
|Human||Not Cited||Ketone bodies stimulate chaperone-mediated autophagy.||Finn PF,Dice JF||The Journal of biological chemistry (280:25864)||2005|
A receptor for the selective uptake and degradation of proteins by lysosomes.
51-2200 was used in Western blot to demonstrate that LAMP-2A/LGP96 overexpression in CHO cells can increase lysosomal proteolytic activity in vitro and in vivo
|Cuervo AM,Dice JF||Science (New York, N.Y.) (273:501)||1996|
Endocytosis of secreted carboxyl ester lipase in a syndrome of diabetes and pancreatic exocrine dysfunction.
51-2200 was used in immunocytochemistry to assess the intracellular distribution of the wild type and mutant carboxyl ester lipase proteins in cellular models
|Torsvik J,Johansson BB,Dalva M,Marie M,Fjeld K,Johansson S,Bjørkøy G,Saraste J,Njølstad PR,Molven A||The Journal of biological chemistry (289:29097)||2014|
|Rat||Not Cited||The p75 neurotrophin receptor evades the endolysosomal route in neuronal cells, favouring multivesicular bodies specialised for exosomal release.||Escudero CA,Lazo OM,Galleguillos C,Parraguez JI,Lopez-Verrilli MA,Cabeza C,Leon L,Saeed U,Retamal C,Gonzalez A,Marzolo MP,Carter BD,Court FA,Bronfman FC||Journal of cell science (127:1966)||2014|
|Human||1:50||A cycling cis-Golgi protein mediates endosome-to-Golgi traffic.||Natarajan R,Linstedt AD||Molecular biology of the cell (15:4798)||2004|
IkappaB is a substrate for a selective pathway of lysosomal proteolysis.
51-2200 was used in Coimmunoprecipitation studies to examine the lysosomal proteolytic pathway of IkB in CHO cells overexpressing LAMP-2
|Cuervo AM,Hu W,Lim B,Dice JF||Molecular biology of the cell (9:1995)||1998|
CD107 antigen-like family member B; CD107b; Igp96; LAMP-2; LAMP-2 Isoform 2A; LAMP-2 Variant A; LAMP-2a; LAMP2; LGP-110; LGP-96; LGP-B; lysosomal membrane glycoprotein 2; lysosomal membrane glycoprotein type B; lysosome-associated membrane glycoprotein 2; lysosome-associated membrane protein 2
CD107b; Lamp II; Lamp-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-B; Mac3