Western blot analysis was performed on whole cell extracts (30 µg lysate) of C2C12 (Lane 1), NIH/3T3 (Lane 2) and PC-12 (Lane 3). The blot was probed with Anti-LAMP2 Rabbit Polyclonal Antibody (Product # 51-2200, 2 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A27036, 0.4µg/ml, 1:2500 dilution). A ~110 kDa band corresponding to LAMP2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by overnight transfer method. The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product# SLF2000S). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide of the cytosolic tail of rat LAMP-2A (GLKRHHTGYEQF)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Inhibition Assays (IA)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody was tested for specificity in rat and mouse (it does not cross-react with the human variant) and was demonstrated to be highly selective for LAMP-2A without cross-reacting with LAMP-2B or C.
This protein was previously known as lgp96.
Lysosome Associated Membrane Protein 2 (LAMP 2) is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. Mature, functional LAMP 2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of ~100-110 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. This protein has 3 variant forms: LAMP-2A, -2B, and 2C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy?
51-2200 was used in western blot to research mitochondrial-targeted catalase good for the old mouse proteome but not the young which may function as a reverse antagonistic pleiotropy
|Basisty N,Dai DF,Gagnidze A,Gitari L,Fredrickson J,Maina Y,Beyer RP,Emond MJ,Hsieh EJ,MacCoss MJ,Martin GM,Rabinovitch PS||Aging cell (15:634)||2016|
Chaperone-Mediated Autophagy after Traumatic Brain Injury.
51-2200 was used in western blot to examine how chaperone-mediated autophagy and the ubiquitin-proteasomal system are regulated after traumatic brain injury.
|Park Y,Liu C,Luo T,Dietrich WD,Bramlett H,Hu B||Journal of neurotrauma (32:1449)||2015|
Degradation of lipid droplet-associated proteins by chaperone-mediated autophagy facilitates lipolysis.
51-2200 was used in western blot to use of chaperone-mediated autophagy to facilitate lipolysis by degradation of lipid droplet-associated proteins
|Kaushik S,Cuervo AM||Nature cell biology (17:759)||2015|
¿-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2.
51-2200 was used in western blot to investigate how endolysosomal dysfunction causes Parkinson's disease-related neurodegeneration
|Kett LR,Stiller B,Bernath MM,Tasset I,Blesa J,Jackson-Lewis V,Chan RB,Zhou B,Di Paolo G,Przedborski S,Cuervo AM,Dauer WT||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:5724)||2015|
PP2A blockade inhibits autophagy and causes intraneuronal accumulation of ubiquitinated proteins.
51-2200 was used in western blot to study the effect of protein phosphatase 2A modulation on neuronal autophagy.
|Magnaudeix A,Wilson CM,Page G,Bauvy C,Codogno P,Lévêque P,Labrousse F,Corre-Delage M,Yardin C,Terro F||Neurobiology of aging (34:770)||2013|
Amphetamine and methamphetamine reduce striatal dopamine transporter function without concurrent dopamine transporter relocalization.
51-2200 was used in western blot to determine dopamine transporter localization following in vivo amphetamine and methamphetamine administration.
|German CL,Hanson GR,Fleckenstein AE||Journal of neurochemistry (123:288)||2012|
Regional deficiencies in chaperone-mediated autophagy underlie ¿-synuclein aggregation and neurodegeneration.
51-2200 was used in western blot to investigate why certain parts of the brain are more vulnerable to protein aggregation.
|Malkus KA,Ischiropoulos H||Neurobiology of disease (46:732)||2012|
|Human||Not Cited||Epidermal growth factor receptor translocation to the mitochondria: regulation and effect.||Demory ML,Boerner JL,Davidson R,Faust W,Miyake T,Lee I,Hüttemann M,Douglas R,Haddad G,Parsons SJ||The Journal of biological chemistry (284:36592)||2009|
|Human||Not Cited||Ketone bodies stimulate chaperone-mediated autophagy.||Finn PF,Dice JF||The Journal of biological chemistry (280:25864)||2005|
A receptor for the selective uptake and degradation of proteins by lysosomes.
51-2200 was used in Western blot to demonstrate that LAMP-2A/LGP96 overexpression in CHO cells can increase lysosomal proteolytic activity in vitro and in vivo
|Cuervo AM,Dice JF||Science (New York, N.Y.) (273:501)||1996|
Pharmacological BACE1 and BACE2 inhibition induces hair depigmentation by inhibiting PMEL17 processing in mice.
51-2200 was used in immunohistochemistry - paraffin section to report that BACE2 and BACE1 inhibition in mice affects melanosome maturation and induces hair depigmentation
|Shimshek DR,Jacobson LH,Kolly C,Zamurovic N,Balavenkatraman KK,Morawiec L,Kreutzer R,Schelle J,Jucker M,Bertschi B,Theil D,Heier A,Bigot K,Beltz K,Machauer R,Brzak I,Perrot L,Neumann U||Scientific reports (6:null)||2016|
Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis.
51-2200 was used in western blot to examine the role of lysosome associated membrane proteins in pancreatitis
|Mareninova OA,Sendler M,Malla SR,Yakubov I,French SW,Tokhtaeva E,Vagin O,Oorschot V,Lüllmann-Rauch R,Blanz J,Dawson D,Klumperman J,Lerch MM,Mayerle J,Gukovsky I,Gukovskaya AS||Cellular and molecular gastroenterology and hepatology (1:678)||2015|
Endocytosis of secreted carboxyl ester lipase in a syndrome of diabetes and pancreatic exocrine dysfunction.
51-2200 was used in immunocytochemistry to assess the intracellular distribution of the wild type and mutant carboxyl ester lipase proteins in cellular models
|Torsvik J,Johansson BB,Dalva M,Marie M,Fjeld K,Johansson S,Bjørkøy G,Saraste J,Njølstad PR,Molven A||The Journal of biological chemistry (289:29097)||2014|
|Not Applicable||Not Cited||
Protective role of endogenous gangliosides for lysosomal pathology in a cellular model of synucleinopathies.
51-2200 was used in immunocytochemistry and western blot to test if altering endogenous ganglioside levels affects lysosomal pathology in a cellular model of synucleinopathy
|Wei J,Fujita M,Nakai M,Waragai M,Sekigawa A,Sugama S,Takenouchi T,Masliah E,Hashimoto M||The American journal of pathology (174:1891)||2009|
Entamoeba histolytica uses ferritin as an iron source and internalises this protein by means of clathrin-coated vesicles.
51-2200 was used in immunocytochemistry to test if trophozoites take up ferritin and use it for their growth in axenic culture
|López-Soto F,González-Robles A,Salazar-Villatoro L,León-Sicairos N,Piña-Vázquez C,Salazar EP,de la Garza M||International journal for parasitology (39:417)||2009|
|Rat||Not Cited||The p75 neurotrophin receptor evades the endolysosomal route in neuronal cells, favouring multivesicular bodies specialised for exosomal release.||Escudero CA,Lazo OM,Galleguillos C,Parraguez JI,Lopez-Verrilli MA,Cabeza C,Leon L,Saeed U,Retamal C,Gonzalez A,Marzolo MP,Carter BD,Court FA,Bronfman FC||Journal of cell science (127:1966)||2014|
|Oculodentodigital dysplasia-causing connexin43 mutants are non-functional and exhibit dominant effects on wild-type connexin43.||Roscoe W,Veitch GI,Gong XQ,Pellegrino E,Bai D,McLachlan E,Shao Q,Kidder GM,Laird DW||The Journal of biological chemistry (280:11458)||2005|
|Human||1:50||A cycling cis-Golgi protein mediates endosome-to-Golgi traffic.||Natarajan R,Linstedt AD||Molecular biology of the cell (15:4798)||2004|
IkappaB is a substrate for a selective pathway of lysosomal proteolysis.
51-2200 was used in Coimmunoprecipitation studies to examine the lysosomal proteolytic pathway of IkB in CHO cells overexpressing LAMP-2
|Cuervo AM,Hu W,Lim B,Dice JF||Molecular biology of the cell (9:1995)||1998|