|Tested species reactivity||Bovine, Human, Mouse, Rat, Xenopus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues G(29) E Q R K D V Y V Q L Y L Q H L T A R N R P(50) of human LAP2 (common to all isoforms).|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25323 detects LAP2 from human samples. MA1-25323 is expected to cross react with mouse (100% conserved), rat (100% conserved), Xenopus laevis (90% conserved), and bovine (88% conserved) due to sequence homology.
MA1-25323 has been successfully used in Western blot procedures.
The MA1-25323 immunogen is a synthetic peptide corresponding to residues G(29) E Q R K D V Y V Q L Y L Q H L T A R N R P(50) of human LAP2 (common to all isoforms).
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B and 1C share a high degree of sequence homology while LAP 2 is distinct from the others. LAP 1A/B bind lamins A, C and B1, while LAP 2 binds lamin B1 only. In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.