MA1-075 has been successfully used in western blot and immunofluorescence applications to detect LAP2 from human and mouse samples.
In western blot, this antibody predominantly detects the LAP2 delta subunit.
The MA1-075 immunogen is full length rat LAP2, and has predicted reactivity with rat samples.
In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B and 1C share a high degree of sequence homology while LAP 2 is distinct from the others. LAP 1A/B bind lamins A, C and B1, while LAP 2 binds lamin B1 only. In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: FLJ31884; HSP86; Hsp89; HSP89A; Hsp90; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; lamina-associated polypeptide 2; Lamina-associated polypeptide 2, isoform alpha; Lamina-associated polypeptide 2, isoform beta; Lamina-associated polypeptide 2, isoforms alpha/zeta; Lamina-associated polypeptide 2, isoforms beta/delta/epsilon/gamma; Lamina-associated polypeptide 2, isoforms beta/gamma; LEM domain containing 4; Splenin; Thymopentin; Thymopoietin; Thymopoietin (lamina associated polypeptide 2); thymopoietin beta; Thymopoietin isoform alpha; Thymopoietin isoform beta; Thymopoietin isoforms alpha/zeta; Thymopoietin isoforms beta/delta/epsilon/gamma; Thymopoietin, isoforms beta/gamma; Thymopoietin-related peptide isoform alpha; Thymopoietin-related peptide isoforms beta/gamma; TP; TP alpha; TP alpha/zeta; TP beta; TP beta/delta/epsilon/gamma; TP beta/gamma; TP5; TPRP isoform alpha; TPRP isoforms beta/gamma
Gene Aliases: 5630400D24Rik; AI195756; AI606875; AW214352; AW547477; CMD1T; LAP2; LEMD4; PRO0868; TMPO; TP