|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||LPS binding site on LBP.|
|Storage buffer||PBS with 0.1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Functional Assay (FN)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-40130 detects LBP from human samples.
MA1-40130 has been successfully used in ELISA,functional assays and immunoprecipitation applications.
The MA1-40130 immunogen is the LPS binding site on LBP.
LPS binding protein (LBP) is an approximately 60 kDa acute phase protein that is produced by hepatocytes. This protein strongly binds to LPS and has been shown to play an important role in the handling of LPS by the host. A number of functions of LBP have been reported. First, LBP transfers LPS to the LPS receptor CD14 on the mononuclear phagocytes, leading to an 100-1,000 fold increased sensitivity of the cells to LPS. Furthermore, LBP can enhance the response of CD14 negitive cells by acceleration of LPS binding to soluble CD14, a complex that stimulates these cells. Next, LBP transfers LPS into High Density Lipoprotein (HDL), which effectively neutralizes its biological potency. LBP was demonstrated to protect mice from septic shock caused by LPS of gram negative bacteria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.