|Tested species reactivity||Bacteria, Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Pig, Rat, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100).|
|Purification||Antigen affinity chromatography|
|Storage buffer||sodium borate|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Immunoprecipitation (IP)||20 µg/500 µg of protein|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody has 92% homology with mouse, 91% with bovine, and 84% with xenopus.
Reacts weaker with mouse than with human samples
LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.