|Tested species reactivity||Bovine, Human, Mouse, Rat, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Genomic sequence made to a N-terminal portion of the human LC3A protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.1% BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:2500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In ICC/IF, autophagosome formation has been seen in HeLa cells after treatment with 50uM chloroquine.
Suggested positive control: human brain lysate, LC3 overexpression lysate.
LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
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