Immunofluorescent analysis of LC3B1+LC3B2 showing staining in the autophagosome of Hep G2 cells. Hep G2 cells mock (left) and treated with 3µM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min and stained using a LC3B1+LC3B2 polyclonal antibody (Product # PA5-32254) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 1 and 125 of Human LC3B|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol, 1% BSA|
|Contains||0.025% Proclin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-32254 targets LC3B1+LC3B2 in WB applications and shows reactivity with Human samples.
The PA5-32254 immunogen is synthetic peptide corresponding to a region within amino acids 1 and 125 of Human LC3B.
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Stimulation of triple negative breast cancer cell migration and metastases formation is prevented by chloroquine in a pre-irradiated mouse model.
PA5-32254 was used in western blot to utilize chloroquine in a pre-irradiated mouse model to prevent cancer cell migration and metastases formation in a simulation of triple negative breast cancer
|Bouchard G,Therriault H,Geha S,Bérubé-Lauzière Y,Bujold R,Saucier C,Paquette B||BMC cancer (16:null)||2016|