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Immunofluorescent analysis of LC3B1+LC3B2 showing staining in the autophagosome of Hep G2 cells. Hep G2 cells mock (left) and treated with 3µM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min and stained using a LC3B1+LC3B2 polyclonal antibody (Product # PA5-32254) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 1 and 125 of Human LC3B|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol, 1% BSA|
|Contains||0.025% Proclin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32254 targets LC3B1+LC3B2 in WB applications and shows reactivity with Human samples.
The PA5-32254 immunogen is synthetic peptide corresponding to a region within amino acids 1 and 125 of Human LC3B.
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ATG8F; autophagy-related protein LC3 B; autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3 beta 2; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like protein
1010001C15Rik; Atg8; ATG8F; ATG8G; LC3B; MAP1A/1BLC3; MAP1ALC3; Map1lc3; MAP1LC3B; MAP1LC3B-a; MAP1LC3B2; Mpl3; zbs559