Immunofluorescent analysis of LCK (p56lck) (green) showing staining in the cytoplasm of Jurkat cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LCK (p56lck) monoclonal antibody (Product # MA5-12303) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Recombinant protein corresponding to aa 1-225 of murine p56lck protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1ug/test|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA5-12303 targets LCK (p56lck) in IP, ICC/IF, FACS and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-12303 immunogen is recombinant protein corresponding to aa 1-225 of mouse p56lck protein.
p56lck is a membrane-associated non-receptor protein tyrosine kinase from the Src-family of kinases The phosphorylation status and the activity of p56lck is regulated by the CD45 tyrosine protein phosphatase. p56lck plays a critical role in T cell development and activation. p56lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Robust fluorescent detection of protein fatty-acylation with chemical reporters.
MA5-12303 was used in immunoprecipitation and western blot to introduce a new method of fluorescent detection of protein fatty-acylation
|Charron G,Zhang MM,Yount JS,Wilson J,Raghavan AS,Shamir E,Hang HC||Journal of the American Chemical Society (131:4967)||2009|