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LDHA Antibody (702747RP488) in ICC/IF
Immunofluorescent analysis of LDHA was performed using 70% confluent PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with LDHA Recombinant Rabbit Monoclonal Antibody (4H18L6), Alexa Fluor™ Plus 488 (Product # 702747RP488, 1:200). Panel a) shows representative images of cells that were stained for detection and localization of LDHA. Panel b) shows representative images of cells stained ... View More
Product Details
702747RP488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
4H18L6
Immunogen
Peptides corresponding to human LDHA [1) aa211-aa229 2) aa2-aa19]
Conjugate
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
proprietary buffer, pH 6.8
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.^M
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Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.^M
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Applications Tested: This 702747RP488 antibody (4H18L6) has been tested by western blot and immunocytochemistry analysis of PC-3 cells. This may be used for immunocytochemistry at 5 µg/ml and for western blotting at less than or equal to 1.25 µg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.^M
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Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser^M
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Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; L-lactate dehydrogenase A chain; Lactate Dehydrogenase; lactate dehydrogenase M; Lactic Dehydrogenase; LDH muscle subunit; LDH-A; LDH-M; OTTHUMP00000165206; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59
Gene Aliases: GSD11; HEL-S-133P; LDHA; LDHM; PIG19
UniProt ID: (Human) P00338
Entrez Gene ID: (Human) 3939
response to hypoxia
lactate metabolic process
pyruvate metabolic process
glycolytic process
response to nutrient
response to glucose
NAD metabolic process
response to drug
response to hydrogen peroxide
positive regulation of apoptotic process
response to estrogen
post-embryonic organ development
response to cAMP
oxidation-reduction process
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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