Immunofluorescent analysis of CD58 (green) showing staining in the cytoplasm of Raji cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD58 monoclonal antibody (Product # MA5800) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Leukocyte Function Antigen-3|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5800 targets CD58 in WB, FACS, IF, IHC(F), IP, and Neu applications and shows reactivity with Human and Mouse samples.
The MA5800 immunogen is human Leukocyte Function Antigen-3.
MA5800 detects CD58 which has a predicted molecular weight of approximately 24 kDa.
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sorting.
MA5800 was used in blocking/activating experiment to investigate the reliability of FACS for the isolation of classical Hodgkin cells
|Fromm JR,Kussick SJ,Wood BL||American journal of clinical pathology (126:764)||2006|
AD2, a human molecule involved in the interaction of T cells with epidermal keratinocytes and thymic epithelial cells.
MA5800 was used in blocking/activating experiment to investigate the importance of AD2 in the interaction between T cells and epidermal keratinocytes and thymic epithelial cells
|Bruggers CS,Patel DD,Scearce RM,Whichard LP,Haynes BF,Singer KH||Journal of immunology (Baltimore, Md. : 1950) (154:2012)||1995|
ICOS-ligand, expressed on human endothelial cells, costimulates Th1 and Th2 cytokine secretion by memory CD4+ T cells.
MA5800 was used in ELISA to investigate the function of ICOS-ligand on human endothelial cells
|Khayyamian S,Hutloff A,Büchner K,Gräfe M,Henn V,Kroczek RA,Mages HW||Proceedings of the National Academy of Sciences of the United States of America (99:6198)||2002|
Human lung microvascular endothelial cells activate allogeneic T cells through an LFA-3-dependent, but CD86-independent mechanism.
MA5800 was used in immunocytochemistry to investigate the mechanism for the allogeneic T cell activation by human lung microvascular endothelial cells
|Hansen AB,Bouchelouche K,Olesen JD||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (109:849)||2001|
CD4+ T cell and eosinophil adhesion is mediated by specific ICAM-3 ligation and results in eosinophil activation.
MA5800 was used in flow cytometry to study the mechanism for the T cell and eosinophil adhesion and activation
|Douglas IS,Leff AR,Sperling AI||Journal of immunology (Baltimore, Md. : 1950) (164:3385)||2000|
Differential expression of cell adhesion molecules CD54/CD11a and CD58/CD2 by human melanoma cells and functional role in their interaction with cytotoxic cells.
MA5800 was used in immunohistochemistry to study the cell adhesion molecules CD54/CD11a and CD58/CD2 in human melanoma cells
|Altomonte M,Gloghini A,Bertola G,Gasparollo A,Carbone A,Ferrone S,Maio M||Cancer research (53:3343)||1993|