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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 51-68 of human LIG1.|
|Storage buffer||PBS with 100µg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: Hela whole cell extract, HeLa cell lysate.
Five distinct DNA ligase activities, I-V, have been identified in mammalian cell extracts. One of these enzymes, LIG1 is central to DNA replication. During DNA replication, the joining of Okazaki fragments by the LIG1 gene product appears to be mediated by an interaction with proliferating cell nuclear antigen (PCNA). LIG1 participates in nucleotide excision and base excision repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ligase I, DNA, ATP-dependent; MGC117397; MGC130025; polydeoxyribonucleotide synthase [ATP] 1