|Immunohistochemistry (Paraffin) (IHC (P))||10 ug/ml|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Lck-interacting membrane protein; Lck-interacting molecule; Lck-interacting transmembrane adapter 1; Lime1
Gene Aliases: dJ583P15.4; LIME; LIME1; LP8067
UniProt ID: (Human) Q9H400
Entrez Gene ID: (Human) 54923
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