|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1/200 - 1/1000|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15453 targets LIN28 in IF, ICC, and WB applications and shows reactivity with human samples.
The MA5-15453 immunogen is purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli.
MA5-15453 detects LIN28 which has a predicted molecular weight of approximately 23kDa.
LIN28: lin-28 homolog (C. elegans), also known as CSDD1, ZCCHC1. Entrez Protein NP_078950. LIN28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.