Invitrogen

LMAN1 Monoclonal Antibody (OTI1A8)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (21) LMAN1 antibodies

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Datasheet

Questions & Answers

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LMAN1 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of LMAN1 was performed using 70% confluent log phase HeLa cells treated with Brefeldin A (5ug/ml, 15 minutes). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with LMAN1 Monoclonal Antibody (OTI1A8) (Product # MA5-25345) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing increase in cytoplasm expression of LMAN1. Panel e represents untreated cells, showing lesser expression of LMAN1. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

LMAN1 Antibody in Immunocytochemistry (ICC) — Immunofluorescent analysis of LMAN1 in COS7 cells. Cells were transfected with a plasmid overexpressing LMAN1 and probed with a LMAN1 monoclonal antibody (Product # MA5-25345).

LMAN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human prostate tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a LMAN1 monoclonal antibody (Product # MA5-25345).

LMAN1 Antibody in Western Blot (WB) — Western blot was performed using Anti-LMAN1 Monoclonal Antibody (OTI1A8) (Product # MA5-25345) and a 58kDa band corresponding to LMAN1 was observed in all tested cell models. Whole cell extracts (30ug) of Hep G2 (Lane 1), HeLa (Lane 2), PANC-1 (Lane 3), MCF7 (Lane 4), Jurkat (Lane 5) and COS-7 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

LMAN1 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with LMAN1 siRNA and reduction of signal was observed in Western Blot using LMAN1 Monoclonal Antibody (OTI1A8) (Product # MA5-25345). {KD}

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:200-1:500

Product Specifications

Species

Dog, Human, Non-human primate, Rat

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1A8

Immunogen

Full length human recombinant protein of LMAN1 produced in HEK293T cell

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2723324

Target Information

The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: endoplasmic reticulum-golgi intermediate compartment protein 53; ER-Golgi intermediate compartment 53 kDa protein; Gp58; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1; lectin, mannose-binding, 1; p58; Protein ERGIC-53

Gene Aliases: ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; LMAN1; MCFD1; MR60; p58

UniProt ID: (Human) P49257, (Rat) Q62902

Entrez Gene ID: (Dog) 476186, (Human) 3998, (Rat) 116666

Molecular Function: membrane traffic protein