OriGene

LMAN1 Monoclonal Antibody (OTI1E3), TrueMAB™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View Details

View all (21) LMAN1 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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LMAN1 Antibody in Immunofluorescence (IF) — Anti-LMAN1 mouse monoclonal antibody (TA502138) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1(RC207088).

LMAN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human lymph node tissue within the normal limits using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502138)

LMAN1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (RC207088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1. Positive lysates LY401709 (100 µg) and LC401709 (20 µg) can be purchased separately from OriGene.

LMAN1 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC207088 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMAN1 antibody (TA502138), and then analyzed by flow cytometry.

LMAN1 Antibody in Knockout — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in LMAN1 knockout (KO) 293T cell line using Anti-LMAN1 monoclonal antibody (Product # CF502138). {KO}

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:500-1:2000

Product Specifications

Species

Dog, Human

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1E3

Immunogen

Full length human recombinant protein of human LMAN1 produced in HEK293T cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: endoplasmic reticulum-golgi intermediate compartment protein 53; ER-Golgi intermediate compartment 53 kDa protein; Gp58; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1; lectin, mannose-binding, 1; Protein ERGIC-53

Gene Aliases: ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; LMAN1; MCFD1; MR60

UniProt ID: (Human) P49257

Entrez Gene ID: (Dog) 476186, (Human) 3998

Molecular Function: membrane traffic protein