|Tested species reactivity||Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||Recombinant human extracellular domain LOX-1 protein from bacteria.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||1:10 - 1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul. In Western blot, this antibody detects intact 50 kDa LOX-1 and also a prominent 32 kDa proteolytic fragment.
LOX-1 (lectin-like oxidized LDL recptor -1) is a 40-50 kDa type II membrane glycoprotein class E scavenger receptor expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoportein (oxLDL), hypochlorite modified high-densidty lipoprotein (HDL), aged /apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.