Immunohistochemistry analysis of Lrp-1b (CD91beta) in mouse E16.5 lung sections. Antigen retrieval from 4% PFA, paraffin embedded sections was performed using EDTA. Following antigen retrieval, tissues were blocked and probed with a Lrp-1b mouse monoclonal antibody (Product # 37-7600) at a dilution of 1:1000. Detection was performed using an Alexa Fluor-568-conjugated anti-mouse secondary antibody (Product # A21124)..
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide derived from the human CD91 beta chain.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
37-7600 was used in IHC to successfully detect LRP-1 in mouse E18.5 lung section.
The protein encoded by this gene is an endocytic receptor involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Breast Cancer MDA-MB-231 Cells Use Secreted Heat Shock Protein-90alpha (Hsp90¿) to Survive a Hostile Hypoxic Environment.
37-7600 was used in western blot to report that heat shock protein-90alpha allows cancer cells to survive hypoxia
|Dong H,Zou M,Bhatia A,Jayaprakash P,Hofman F,Ying Q,Chen M,Woodley DT,Li W||Scientific reports (6:null)||2016|
Hsp90α and Hsp90β together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing.
37-7600 was used in western blot to elucidate a role for heat shock proteins in ischemia.
|Jayaprakash P,Dong H,Zou M,Bhatia A,O'Brien K,Chen M,Woodley DT,Li W||Journal of cell science (128:1475)||2015|