Invitrogen

LRP1 Monoclonal Antibody (8G1)

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Published Figures (2)

FIGURE: 1 / 2

LRP1 Antibody (MA1-27198) in Flow

Oncoimmunology 2016 - Figure 4. Human DCs take up platinum-treated tumor cells in a CRT-dependent manner. ( A ) Percentage of uptake of 15 uM OXP or CDDP treated-BLM cells by CD1c + DCs. Control or treated tumor cells were co-cultured with DCs in the presence of isotype (white bar), CRT blocking peptide (gray bar) or irrelevant tumor antigen (gp100) peptide (black bar) for 2 h. Extent of uptake was assessed by flow cytometry. Da... View More

Product Image Gallery

LRP1 Antibody in Neutralization (Neu) — Fig. 3 MDK activates T cells to produce Ccl4 through Lrp1/calcineurin/NFAT1 signaling. a T cells expressed only two (Lrp1 and Lrp6) of the putative MDK receptors [protein-tyrosine phosphatase zeta ( Ptprz1 ), neuroglycan-C ( Cspg5 ), low density-lipoprotein receptor-related protein-1 ( Lrp1 ), low density-lipoprotein receptor-related protein-6 ( Lrp6 ) and anaplastic lymphoma kinase ( Alk )] by quantitative RT-PCR. Normal mouse cortex was used as an internal positive control. b Lrp1 blocking antibodies (30 ug ml -1 ) reduced MDK-induced Ccl4 production in T cells. c The 2041C>T neuron conditioned media (N-CM)-mediated Ccl4 production in T cells was attenuated following exposure to Lrp1 receptor blocking antibodies. d Immunoblotting revealed increased NFAT1 nuclear localization in T cells following MDK treatment. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and TATA-binding protein (TBP) served as loading controls for the cytoplasm and nuclear fractions, respectively. e Decreased levels of phosphorylated-NFAT1 (p-NFAT) were observed after MDK stimulation of T cells. Lrp1 blocking antibodies (anti-Lrp1, 30 ug ml -1 ) increased NFAT1 phosphorylation and impaired NFAT1 nuclear localization in MDK-stimulated T cells. f The calcineurin inhibitor FK506 (10 muM) inhibited MDK-induced NFAT1 nuclear localization. g Calcineurin inhibitors, cyclosporin (100 nM) and FK506 (10 muM), inhibited MDK-induced Ccl4 production in T cells. h FK506 (10 muM) reduced 2041C>T neuron conditioned me

Product Details

MA1-27198

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1-10 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

1-10 µg/mL

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Neutralization (Neu)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8G1

Immunogen

Human LRP/alpha2 MR.

Conjugate

Unconjugated

Form

Liquid

Concentration

2.0 mg/mL

Purification

Protein A

Storage buffer

0.05M sodium phosphate, pH 7.4, with 0.15M NaCl

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_794778

Product Specific Information

Perform Western blot analysis under non-reducing conditions.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is an endocytic receptor involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Givinostat-Liposomes: Anti-Tumor Effect on 2D and 3D Glioblastoma Models and Pharmacokinetics.

Cancers

Taiarol L,Bigogno C,Sesana S,Kravicz M,Viale F,Pozzi E,Monza L,Carozzi VA,Meregalli C,Valtorta S,Moresco RM,Koch M,Barbugian F,Russo L,Dondio G,Steinkühler C,Re F

MA1-27198 was used in Western Blotting to provide the proof of concept for a delivery system that can improve the in vivo half-life and increase the brain delivery of Givinostat, a pan-HDAC inhibitor.

Thu Jun 16 00:00:00 EDT 2022

Midkine activation of CD8⁺ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth.

Nature communications

Guo X,Pan Y,Xiong M,Sanapala S,Anastasaki C,Cobb O,Dahiya S,Gutmann DH

MA1-27198 was used in Neutralization to define the axis in which neurons, T cells, and microglia interact to govern Neurofibromatosis-1 (NF1) low-grade glioma (LGG) growth.

Fri May 01 00:00:00 EDT 2020

Midkine activation of CD8⁺ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth.

Nature communications

Guo X,Pan Y,Xiong M,Sanapala S,Anastasaki C,Cobb O,Dahiya S,Gutmann DH

MA1-27198 was used in Neutralization to define the axis in which neurons, T cells, and microglia interact to govern Neurofibromatosis-1 (NF1) low-grade glioma (LGG) growth.

Fri May 01 00:00:00 EDT 2020

Human CD1c(+) DCs are critical cellular mediators of immune responses induced by immunogenic cell death.

Oncoimmunology

Di Blasio S,Wortel IM,van Bladel DA,de Vries LE,Duiveman-de Boer T,Worah K,de Haas N,Buschow SI,de Vries IJ,Figdor CG,Hato SV

MA1-27198 was used in flow cytometry investigate the impact of CD1c positive dendritic cells on immunogenic cell death

Mon Aug 01 00:00:00 EDT 2016

Plasma Low-density Lipoprotein Receptor-related Protein 1 Concentration is not Associated with Human Abdominal Aortic Aneurysm Presence.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery

Moxon JV,Behl-Gilhotra R,Morton SK,Krishna SM,Seto SW,Biros E,Nataatmadja M,West M,Walker PJ,Norman PE,Golledge J

MA1-27198 was used in blocking or activating experiment to study the role of low-density lipoprotein receptor-related protein 1 in abdominal aortic aneurysm

Thu Oct 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: A2MR; Alpha-2-macroglobulin receptor; APOER; Apolipoprotein E receptor; CD91; FLJ16451; low density lipoprotein receptor-related protein 1; Low-density lipoprotein receptor-related protein 1 515 kDa subunit; Low-density lipoprotein receptor-related protein 1 85 kDa subunit; Low-density lipoprotein receptor-related protein 1 intracellular domain; LRP-1; LRP-515; LRP-85; LRPICD; MGC88725; Prolow-density lipoprotein receptor-related protein 1; TbetaR-V/LRP-1/IGFBP-3 receptor; type V tgf-beta receptor

View more View less

Gene Aliases: A2MR; APOER; APR; CD91; IGFBP3R; LRP; LRP1; LRP1A; TGFBR5

View more View less

UniProt ID: (Human) Q07954

View more View less

Entrez Gene ID: (Human) 4035

View more View less

Molecular Function: apolipoprotein extracellular matrix structural protein transmembrane signal receptor

Function(s)

retinoid metabolic process receptor-mediated endocytosis protein kinase C-activating G-protein coupled receptor signaling pathway aging cell proliferation positive regulation of cholesterol efflux negative regulation of neuron projection development negative regulation of smooth muscle cell migration cerebral cortex development negative regulation of Wnt signaling pathway positive regulation of lipid transport regulation of cholesterol transport regulation of phospholipase A2 activity regulation of actin cytoskeleton organization aorta morphogenesis lipoprotein metabolic process lipoprotein transport apoptotic cell clearance negative regulation of neuron apoptotic process positive regulation of protein transport beta-amyloid clearance negative regulation of platelet-derived growth factor receptor-beta signaling pathway

Process(es)

protease binding receptor activity low-density lipoprotein receptor activity calcium ion binding protein binding protein complex binding apolipoprotein binding lipoprotein transporter activity poly(A) RNA binding lipoprotein particle receptor binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

LRP1 Monoclonal Antibody (8G1)

LRP1 Antibody (MA1-27198) in Flow

Product Details

MA1-27198

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!