Invitrogen

LRP1 Recombinant Rabbit Monoclonal Antibody (SA0290)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Reference

View all (29) LRP1 (CD91) antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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LRP1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of LRP1 in MCF-7 cells using a LRP1 Monoclonal antibody (Product # MA5-31959) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

LRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of LRP1 of paraffin-embedded Mouse brain tissue using a LRP1 Monoclonal antibody (Product #MA5-31959). Counter stained with hematoxylin.

LRP1 Antibody in Western Blot (WB) — Western blot was performed using Anti-LRP1 Recombinant Rabbit Monoclonal Antibody (Product # MA5-31959) and a 85 kDa band corresponding to LRP1 was observed in cell lines and tissues tested except SK-BR-3 cell line. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), MDA-MB-231 (Lane 2), SK-BR-3 (Lane 3), HeLa (Lane 4), Mouse Liver (Lane 5) and Mouse Heart (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2500 dilution) and detected by Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

LRP1 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of LRP1 in Hela cells using a LRP1 Monoclonal Antibody (Product # MA5-31959) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

LRP1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of LRP1 was observed among the breast cancer cell lines: MDA-MB 231, a cell model positive for LRP1 expression, in comparison to SK-BR-3, a negative cell model for LRP1 expression using Anti-LRP1 Recombinant Rabbit Monoclonal Antibody (Product # MA5-31959) in western blot. Relative expression was also observed across mouse tissues tested, where LRP1 is highly expressed in liver as compared to heart. {RE}

Product Details

MA5-31959

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	1:200 - 1:2,000	-
Immunocytochemistry (ICC/IF)	1:50	-
Flow Cytometry (Flow)	1:50	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	SA0290
Immunogen	Synthetic peptide within Human LRP1 aa 4,471-4,520 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809253

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The protein encoded by this gene is an endocytic receptor involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CREBH normalizes dyslipidemia and halts atherosclerosis in diabetes by decreasing circulating remnant lipoproteins.

The Journal of clinical investigation

Shimizu-Albergine M,Basu D,Kanter JE,Kramer F,Kothari V,Barnhart S,Thornock C,Mullick AE,Clouet-Foraison N,Vaisar T,Heinecke JW,Hegele RA,Goldberg IJ,Bornfeldt KE

MA5-31959 was used in Western Blot to study how CREBH is influenced through APOE-dependent pathways in a mouse-model of type 1 diabetes mellitus.

Mon Nov 15 00:00:00 EST 2021

Bioinformatics

Protein Aliases: A2MR; alpha 2-macroglobulin receptor; Alpha-2-macroglobulin receptor; APOER; Apolipoprotein E receptor; CD91; FLJ16451; lipoprotein receptor-related protein; low density lipoprotein receptor-related protein 1; low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor); Low-density lipoprotein receptor-related protein 1 515 kDa subunit; Low-density lipoprotein receptor-related protein 1 85 kDa subunit; Low-density lipoprotein receptor-related protein 1 intracellular domain; LRP-1; LRP-515; LRP-85; lrp1; LRPICD; MGC88725; Prolow-density lipoprotein receptor-related protein 1; TbetaR-V/LRP-1/IGFBP-3 receptor; type V tgf-beta receptor

Gene Aliases: A2MR; AI316852; APOER; APR; b2b1554Clo; CD91; IGFBP3R; LRP; LRP1; LRP1A; TGFBR5

UniProt ID: (Human) Q07954, (Mouse) Q91ZX7, (Rat) G3V928

Entrez Gene ID: (Human) 4035, (Mouse) 16971, (Rat) 299858

Molecular Function: apolipoprotein extracellular matrix structural protein transmembrane signal receptor