Immunohistochemistry analysis of ApoER2 / LRP8 showing staining in the membrane of paraffin-embedded human placenta tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a ApoER2 / LRP8 Rabbit Polyclonal Antibody (40-7800) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of the human ApoER2 protein, which differs from mouse by two non-conservative amino acid replacements|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Apolipoprotein E receptor 2 (ApoER2) is an NMDA associated protein found in neurons. It participates in important functions from guiding neurons in the embryonic brain to regulating synaptic signaling. It is also involved in debilitating neurodegenerative diseases such as schizophrenia and Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||Efficient cytosolic delivery mediated by polymersomes facilely prepared from a degradable, amphiphilic, and amphoteric copolymer.||Huang Z,Teng W,Liu L,Wang L,Wang Q,Dong Y||Nanotechnology (24:null)||2013|
|Rat||Not Cited||Efficient cytosolic delivery mediated by polymersomes facilely prepared from a degradable, amphiphilic, and amphoteric copolymer.||Huang Z,Teng W,Liu L,Wang L,Wang Q,Dong Y||Nanotechnology (24:null)||2013|