OriGene

LSM1 Monoclonal Antibody (OTI5C6), TrueMAB™

View all (20) LSM1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

LSM1 Antibody in Immunocytochemistry (ICC/IF) — Anti-LSM1 mouse monoclonal antibody (TA503120°C Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1(RC200288).

LSM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human Kidney tissue within the normal limits using anti-LSM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503120°C

LSM1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (RC200288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1. Positive lysates LY415265 (100 µg) and LC415265 (20 µg) can be purchased separately from OriGene.

LSM1 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC200288 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody (TA503120°C, and then analyzed by flow cytometry.

Product Details

CF503120

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

1:100

Product Specifications

Species Reactivity

Dog, Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI5C6

Immunogen

Full length human recombinant protein of human LSM1 produced in HEK293T cell.

View immunogen

Conjugate

Unconjugated

Form

lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cancer-associated Sm protein; Cancer-associated Sm-like; cancer-associated Sm-like protein; cancer‐associated Sm protein; LSM1 homolog, U6 small nuclear RNA associated; LSM1 mRNA degradation associated; LSM1, U6 small nuclear RNA associated; LSM1-like protein U6 small nuclear RNA associated; Small nuclear ribonuclear CaSm; U6 snRNA-associated Sm-like protein LSm1

Gene Aliases: CASM; LSM1; YJL124C

UniProt ID: (Human) O15116

Entrez Gene ID: (Dog) 475587, (Human) 27257

Molecular Function: RNA splicing factor

Function(s)

deadenylation-dependent decapping of nuclear-transcribed mRNA RNA splicing, via transesterification reactions mRNA processing RNA splicing stem cell population maintenance exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay negative regulation of neuron differentiation histone mRNA catabolic process

Process(es)

RNA cap binding mRNA binding protein binding pre-mRNA binding poly(A) RNA binding