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LTA4H Monoclonal Antibody (OTI7D11), TrueMAB™

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Protocols

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LTA4H Antibody in Immunocytochemistry (ICC/IF) — Anti-LTA4H mouse monoclonal antibody (TA500643) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LTA4H(RC207617).

LTA4H Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human prostate tissue within the normal limits using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500643)

LTA4H Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LTA4H (RC207617, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-LTA4H. Positive lysates LY424467 (100 µg) and LC424467 (20 µg) can be purchased separately from OriGene.

LTA4H Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC207617 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LTA4H antibody (TA500643), and then analyzed by flow cytometry.

LTA4H Antibody in Immunoprecipitation (IP) — Immunoprecipitation of LTA4H by using TrueMab monoclonal anti-LTA4H antibody (Negative control: IP without adding anti-LTA4H antibody). For each experiment, 500ul of tagged LTA4H overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of ant-LTA4H antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Product Details

CF500643

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-
Immunoprecipitation (IP)	2-4 µg/mg	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI7D11
Immunogen	Full length human recombinant protein of human LTA4H produced in HEK293T cell. View immunogen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). The enzyme also has some peptidase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Leukotriene A(4) hydrolase; Leukotriene A-4 hydrolase; LKHA4; LTA-4 hydrolase; testicular secretory protein Li 27; Tripeptide aminopeptidase LTA4H

Gene Aliases: LTA4; LTA4H

UniProt ID: (Human) P09960

Entrez Gene ID: (Human) 4048

Function(s)

proteolysis leukotriene metabolic process response to zinc ion leukotriene biosynthetic process peptide catabolic process response to peptide hormone Type I pneumocyte differentiation

Process(es)

aminopeptidase activity epoxide hydrolase activity leukotriene-A4 hydrolase activity protein binding peptidase activity zinc ion binding peptide binding poly(A) RNA binding metalloaminopeptidase activity

LTA4H Monoclonal Antibody (OTI7D11), TrueMAB™

Product Details

CF500643

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

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